Oligonucleotide-based Biosenser for Highly Sensitive Detection of Hg<sup>2+</sup> in Aqueous Solution

doi:10.13523/j.cb.2212011

China Biotechnology

2023, Vol. 43

Issue (7): 53-59 DOI: 10.13523/j.cb.2212011

Oligonucleotide-based Biosenser for Highly Sensitive Detection of Hg²⁺ in Aqueous Solution

Li-ping MA,Yun-xia LI,Ying-ying NIE,Sheng-long MA,Bo ZHENG^**(

)

Institute of Sensor Technology, Gansu Academy of Sciences, Key Laboratory of Sensor and Sensing Technology of Gansu, Lanzhou 730099, China

Download:

HTML

PDF(1707KB) HTML
Export: BibTeX | EndNote (RIS)

Abstract

Objective: Mercury is a highly toxic and widespread pollutant in the environment, which can cause harm to human body even in low concentration. Based on the T-Hg²⁺-T base mismatch principle, a new biosensor was constructed to detect the concentration of Hg²⁺ in water with high sensitivity and selectivity. Methods: A new Gelred/TRO biosensor was constructed by using thymine rich oligonucleotide (TRO) as the specific Hg²⁺ recognition probe, and Gelred as the fluorescence indicator probe. The sensor is used to detect Hg²⁺ in water. The influence of many factors on the detection system was studied. Results: Under the optimized detection conditions, the linear range of Hg²⁺ detection was 10 ~ 800 nmol/L, which was fitted as follows: y=0.005 96x+0.881 44 (r²=0.991 47). The detection limit was 0.14 nmol/L. The presence of ten interfering ions such as Fe²⁺, Co²⁺, Cu²⁺, K⁺, Mg²⁺, Ca²⁺, Ag⁺, Cl^-, $SO 4 2 -$ , and $NO 3 -$ did not affect the detection of Hg²⁺. The average recovery of Hg²⁺ in tap water samples and the Yellow River were 98.08% and 95.1%, respectively. Conclusion: This method has the advantages of simple operation, high sensitivity, good specificity and low detection limit. It has a good application prospect in the rapid determination of Hg²⁺ in drinking water.

Key words： Oligonucleotide Gelred Biosenser Fluorescent Hg²⁺

Received: 05 December 2022 Published: 03 August 2023

ZTFLH:

O657.1

	Service
	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	Li-ping MA
	Yun-xia LI
	Ying-ying NIE
	Sheng-long MA
	Bo ZHENG

Cite this article:

Li-ping MA, Yun-xia LI, Ying-ying NIE, Sheng-long MA, Bo ZHENG. Oligonucleotide-based Biosenser for Highly Sensitive Detection of Hg²⁺ in Aqueous Solution. China Biotechnology, 2023, 43(7): 53-59.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.2212011 OR https://manu60.magtech.com.cn/biotech/Y2023/V43/I7/53

Fig.1

Fig.2 Fluorescence spectra of solution in the presence and absence of Hg²⁺ (a) Response of Gelred solution to Hg²⁺ (b) Response of Gelred/TRO sensor to Hg²⁺

Fig.3 The fluorescence intensity changes depending on the concentration of TRO

Fig.4 Effect of pH value on fluorescence intensity of sensor

Fig.5 Effect of different reaction times on fluorescence intensity of sensor

Fig.6 Fluorescence spectra of different concentrations of Hg²⁺ detected by Gelred/TRO sensor

Fig.7 Linear relationship between fluorescence intensity and Hg²⁺concentrations

Fig.8 Specificity analysis of Gelred/TRO sensor detection of Hg²⁺

Table 1 Detection results of different concentrations of Hg²⁺ in tap water and the Yellow River


[1]	Sellaoui L, Soetaredjo F E, Ismadji S, et al. Equilibrium study of single and binary adsorption of lead and mercury on bentonite-alginate composite: experiments and application of two theoretical approaches. Journal of Molecular Liquids, 2018, 253: 160-168. doi: 10.1016/j.molliq.2018.01.056

[2]	Kokab T, Shah A, Iftikhar F J, et al. Amino acid-fabricated glassy carbon electrode for efficient simultaneous sensing of zinc(II), cadmium(II), copper(II), and mercury(II) ions. ACS Omega, 2019, 4(26): 22057-22068. doi: 10.1021/acsomega.9b03189 pmid: 31891086

[3]	Chiang C K, Huang C C, Liu C W, et al. Oligonucleotide-based fluorescence probe for sensitive and selective detection of mercury(II) in aqueous solution. Analytical Chemistry, 2008, 80(10): 3716-3721. doi: 10.1021/ac800142k

[4]	Zhang Y K, Yan T, Yan L G, et al. Preparation of novel cobalt ferrite/chitosan grafted with graphene composite as effective adsorbents for mercury ions. Journal of Molecular Liquids, 2014, 198: 381-387. doi: 10.1016/j.molliq.2014.07.043

[5]	Khani H, Abbasi S, Tavakkoli Yaraki M, et al. A naked-eye colorimetric assay for detection of Hg2+ ions in real water samples based on gold nanoparticles-catalyzed clock reaction. Journal of Molecular Liquids, 2022, 345: 118243. doi: 10.1016/j.molliq.2021.118243

[6]	中华人民共和国卫生部, 中国国家标准化管理委员会. 生活饮用水卫生标准: GB 5749-2006. 北京: 中国标准出版社 2007.

[6]	Ministry of Health of the People’s Republic of China, Standardization Administration of the People’s Republic of China. Standards for drinking water quality: GB 5749-2006. Beijing: Standards Press of China, 2007.

[7]	World Health Organization. Guidelines for drinking-water quality. Geneva: WHO, 2011.

[8]	Salar Amoli H, Porgam A, Bashiri Sadr Z, et al. Analysis of metal ions in crude oil by reversed-phase high performance liquid chromatography using short column. Journal of Chromatography A, 2006, 1118(1): 82-84. pmid: 16723133

[9]	Bettmer J, Heilmann J, Kutscher D J, et al. Direct μ-flow injection isotope dilution ICP-MS for the determination of heavy metals in oil samples. Analytical and Bioanalytical Chemistry, 2012, 402(1): 269-275. doi: 10.1007/s00216-011-5420-6 pmid: 21983977

[10]	Cai W, Xie S B, Zhang J, et al. An electrochemical impedance biosensor for Hg2+ detection based on DNA hydrogel by coupling with DNA zyme-assisted target recycling and hybridization chain reaction. Biosensors and Bioelectronics, 2017, 98(15): 466-472. doi: 10.1016/j.bios.2017.07.025

[11]	Ebrahimi M, Raoof J B, Ojani R. Sensitive electrochemical DNA-based biosensors for the determination of Ag+ and Hg2+ ions and their application in analysis of amalgam filling. Journal of the Iranian Chemical Society, 2018, 15(8): 1871-1880. doi: 10.1007/s13738-018-1384-1

[12]	Yu Y J, Yu C, Gao R F, et al. Dandelion-like CuO microspheres decorated with Au nanoparticle modified biosensor for Hg2+ detection using a T-Hg2+-T triggered hybridization chain reaction amplification strategy. Biosensors and Bioelectronics, 2019, 131(15): 207-213. doi: 10.1016/j.bios.2019.01.063

[13]	Miao P, Tang Y G, Wang L. DNA modified Fe3O4@Au magnetic nanoparticles as selective probes for simultaneous detection of heavy metal ions. ACS Applied Materials & Interfaces, 2017, 9(4): 3940-3947.

[14]	Torigoe H, Ono A, Kozasa T. HgII ion specifically binds with T: T mismatched base pair in duplex DNA. Chemistry - A European Journal, 2010, 16(44): 13218-13225. doi: 10.1002/chem.201001171

[15]	Sohrabi H, Khataee A, Ghasemzadeh S, et al. Layer double hydroxides (LDHs)- based electrochemical and optical sensing assessments for qua.pngication and ide.pngication of heavy metals in water and environment samples: a review of status and prospects. Trends in Environmental Analytical Chemistry, 2021, 31: e00139. doi: 10.1016/j.teac.2021.e00139

[16]	Huang Y Z, Li L, Zhang Y, et al. Auto-cleaning paper-based electrochemiluminescence biosensor coupled with binary catalysis of cubic Cu2O-Au and polyethyleneimine for qua.pngication of Ni2+ and Hg2+. Biosensors and Bioelectronics, 2019, 126: 339-345. doi: 10.1016/j.bios.2018.11.008

[17]	Xing Y P, Xue B Y, Qi P S, et al. A rapid and sensitive fluorescence biosensor for Hg2+ detection in environmental samples. Sensors and Actuators Reports, 2022, 4: 100101. doi: 10.1016/j.snr.2022.100101

[18]	Karthikeyan K, Sujatha L. Fluorometric sensor for mercury ion detection in a fluidic MEMS device. IEEE Sensors Journal, 2018, 18(13): 5225-5231. doi: 10.1109/JSEN.2018.2840331

[19]	Li Y, Liu N, Liu H, et al. A novel label-free fluorescence assay for one-step sensitive detection of Hg2+ in environmental drinking water samples. Scie.pngic Reports, 2017, 7: 45974.

[20]	Qiu H Z, Wu N M, Zheng Y H, et al. A robust and versatile signal-on fluorescence sensing strategy based on SYBR green I dye and graphene oxide. International Journal of Nanomedicine, 2014, 10: 147-156.

[21]	Yang F, Zhao M L, Zheng B Z, et al. Influence of pH on the fluorescence properties of graphene quantum dots using ozonation pre-oxide hydrothermal synthesis. Journal of Materials Chemistry, 2012, 22(48): 25471-25479. doi: 10.1039/c2jm35471c

[22]	Wang R Y, Zhou X H, Shi H C, et al. T-T mismatch-driven biosensor using triple functional DNA-protein conjugates for facile detection of Hg2+. Biosensors and Bioelectronics, 2016, 78: 418-422. doi: 10.1016/j.bios.2015.11.082

[1]	ZHU Shu,ZHU Li-feng,YANG Shuo,JIN Ran,WANG Yu-sheng,SUN Bao-quan. Applications of Silicon Nanowires in Biosensors:A Review[J]. China Biotechnology, 2022, 42(1/2): 174-181.

[2]	HU Xuan,WANG Song,YU Xue-ling,ZHANG Xiao-peng. Construction of a Destabilized EGFP Cell Model for Gene Editing Evaluation[J]. China Biotechnology, 2021, 41(5): 17-26.

[3]	Cheng-cheng ZHAO,Chang-po SUN,Xiao-jiao CHANG,Song-ling WU,Zhen-quan LIN. Construction and Application of Cell Lysis Systems in the Expression of Mycotoxin Degrading Enzyme in Escherichia coli[J]. China Biotechnology, 2019, 39(4): 69-77.

[4]	Kai-yun MAO,Da-ming CHEN,Yue-lei FAN,Yue WANG,Hong-bo JIANG. Development Status and Trend Analysis of Oligonucleotide Therapies[J]. China Biotechnology, 2018, 38(4): 96-106.

[5]	Yuan-qiao CHEN,Ding-pei LONG,Xiao-xue DOU,Run QI,Ai-chun ZHAO. Studies on the Protein Purification Ability of an ELP₃₀-Tag in Prokaryotic Expression System[J]. China Biotechnology, 2018, 38(2): 54-60.

[6]	FU Li-wen, ZHANG Yu, YI Han, LI Xue, ZHU Nai-shuo. Establishment and Application of Multiplex Fluorescent Real-time PCR for Detecting Six Kinds of Animal Derived Materials[J]. China Biotechnology, 2017, 37(9): 48-59.

[7]	Yan-yan LIU,Hui-rong LI,Yue HU,Yang-yang FAN,Xiang-ming LI,Qing-qing TAN,Jia-qiang WU,Xun BU. Multiplex Fluorescent Real-time PCR Detection of Fox, Mink, Raccoon and Dog Derived Materials in Feedstuff[J]. China Biotechnology, 2017, 37(12): 67-76.

[8]	REN Shuang, ZHU Hong-liang. Establishment of Taqman Quantitative PCR System to Estimate Copy Numbers of Exogenous Transgene in Genome Edited Tomato[J]. China Biotechnology, 2017, 37(10): 72-80.

[9]	WANG Hong-su, GUAN Gui-jing, LIU Jin-xiang. Application of Alexa Fluor in Cytology and Molecular Biology[J]. China Biotechnology, 2015, 35(9): 71-77.

[10]	WEI Yan, WANG Huan-qin, WU Meng, ZHANG Feng-juan, LIANG Guo-dong, ZHU Wu-yang. Construction and Identification of the Cell Line for Detecting Flaviviruses[J]. China Biotechnology, 2015, 35(9): 35-41.

[11]	ZHOU Yan, WANG Xiao-nan, YAN Shan-shan, WU Jin-yuan, SUN Mao-sheng, ZHANG Lei, LI Hong-jun. Construction and Coexpression of Recombinant Adenovirus Containing EGFP and Lmx1A[J]. China Biotechnology, 2014, 34(9): 72-79.

[12]	ZHAO Hai-yang, WANG Ze, HUANG Peng-huang, TIAN Hai-shan, LI Xiao-kun, YANG Shu-lin. rhKGF2-EGFP Fusion Expression and Alcohol Liposome Preparation[J]. China Biotechnology, 2014, 34(10): 22-27.

[13]	TIAN Ting, CHANG Jian, ZHANG Xin, JIANG Chen-yu, ZHANG Yun-hai, LIU Xiao-mei, ZHANG Chun. In Vivo Imaging of Near-infrared Fluorescent Protein in Skeletal Muscle of Mice Mediated by Recombinant Adeno-associated Virus[J]. China Biotechnology, 2014, 34(10): 67-72.

[14]	YI Min, LÜ Pin, WU Li-hua, YI Hui-lan. Construction of an Engineered Yeast with Green Fluorescent Protein Gene and Its Fluorescence in Response to Copper Ion[J]. China Biotechnology, 2012, 32(10): 14-18.

[15]	SHI Yong-qian, HE Wen-teng, ZHOU Yang, JIAO Ming-xia, XIE Bing-teng, HU Kui, KONG Qing-ran, LIU Zhong-hua. Study on Generation of Chimeric Porcine Embryos Derived from Lentivirus Mediated Green Fluorescent Protein(GFP) Transgene Embryos[J]. China Biotechnology, 2012, 32(08): 68-74.

Viewed

Full text

Abstract

Cited

Shared

Discussed