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Production of Single-chain Fragment Variable and Its Application in Tumor Diagnosis and Treatment |
LIN Peng1,2,QIAN Jing1,2,3,FENG Qiang2,LEI Jing2,JIANG Ya2,YANG Ju-lun2,***() |
1 School of Medicine, Kunming University of Science and Technology, Kunming 650500, China 2 Department of Pathology, 920th Hospital of the Joint Logistics Support Force of PLA, Kunming 650032, China 3 School of Life Sciences, Kunming University of Science and Technology, Kunming 650032, China |
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Abstract Single-chain fragment variable (scFv) is a small molecule recombinant antibody consisting of a variable heavy chain (VH) and a variable light chain (VL) linked together by a flexible peptide junction. The mRNA of single chain antibodies is isolated mainly from hybridomas and reverse transcribed into cDNA as a template for scFv gene amplification, resulting in a gene library containing a large number of different VH and VL fragments of scFv. Screening and identification of the affinity and specificity of the scFv is accomplished using different display techniques, and the resulting scFv can be used to successfully express its proteins through various expression systems. Despite its small molecular weight, scFv contains the antigen-binding domain of intact antibodies and has high specificity and affinity for antigens, as well as low immunogenicity, and also has a strong ability to penetrate and spread through tumor tissue. Therefore, scFv has become a hot research topic in the development of oncology therapeutic approaches. This review details the methods and problems in the preparation of scFv and focuses on the research progress of scFv in tumor diagnosis and treatment, with a view to providing a theoretical basis for the preparation of scFv and its application in diagnosis and treatment of tumors.
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Received: 18 January 2023
Published: 04 July 2023
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