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Analysis of Differentially Expressed Proteins in the Cervical Spinal Cord of Guinea Pigs Subacutely Exposed to Soman |
JIN Qian,SHI Meng,LIU Zhan-biao,ZHANG Yi,ZHU Si-qing,SHI Jing-jing,ZONG Xing-xing,CHEN Xue-jun*(),LI Li-qin*() |
State Key Laboratory of NBC Protection for Civilians, Beijing 102205, China |
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Abstract Objective: Proteomic technique was used to study the differentially expressed proteins in the cervical spinal cord of guinea pigs subacutely exposed to soman, to explore the main biological pathways affected by soman poisoning, and to provide important biomarkers for the diagnosis, treatment, and prognosis of soman poisoning. Methods: Soman (0.2×LD50) was subcutaneously injected into the back of male adult guinea pigs once a day for 14 consecutive days for subacute exposure. The cervical spinal cord tissue was collected after the final exposure, and the differentially expressed proteins between the soman and control group were analyzed by proteomics technology. Functional annotation and pathway enrichment analysis of differentially expressed proteins was performanced using KEGG database. The function of differential proteins in important pathways was analyzed and discussed. Results: A total of 3 563 proteins annotated by KEGG database were identified in cervical spinal cord tissues of guinea pigs. Principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) showed that there were 407 differentially expressed proteins after exposure to soman, of which 276 proteins were up-regulated and 131 were down-regulated. These differential proteins are mainly related to metabolism (M), genetic information processing (GIP), environmental information processing (EIP), cellular processes (CP), organismal systems (OS), and human diseases (HD). The up-regulated proteins are mainly enriched in 27 pathways (P<0.05), and the down-regulated proteins are mainly enriched in 8 pathways (P<0.05). The main enrichment pathways of up-regulated proteins include phagosome pathway, tight junction pathway, extracellular matrix (ECM)-receptor interaction pathway, PI3K-Akt signaling pathway, JAK-STAT signaling pathway, complement and coagulation cascade pathway, which are related to up regulation of 24 protein expressions. Conclusion: Based on proteomics research, it was clarified that a large number of differentially expressed proteins appeared in the cervical spinal cord of guinea pigs subacutely exposed to soman, and the six main pathways were helpful to further elucidate the non-cholinergic mechanism of subacute injury. The differential expressed proteins could provide important biomarkers for the diagnosis, treatment, and prognosis of poisoning, as well as important theoretical basis for the development of new antitoxic drugs.
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Received: 26 September 2022
Published: 31 March 2023
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Corresponding Authors:
*Xue-jun CHEN,Li-qin LI
E-mail: chenxuejun86@sina.com;llq969696@163.com
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Cite this article:
JIN Qian, SHI Meng, LIU Zhan-biao, ZHANG Yi, ZHU Si-qing, SHI Jing-jing, ZONG Xing-xing, CHEN Xue-jun, LI Li-qin. Analysis of Differentially Expressed Proteins in the Cervical Spinal Cord of Guinea Pigs Subacutely Exposed to Soman. China Biotechnology, 2023, 43(2/3): 64-74.
URL:
https://manu60.magtech.com.cn/biotech/10.13523/j.cb.2209066 OR https://manu60.magtech.com.cn/biotech/Y2023/V43/I2/3/64
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