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Propagation and Preservation of Germplasm Resources in Pinellia ternata (Thunb.)Breit. |
JIA Ming-liang1,FANG He-fang1,**(),ZHANG Ben-hou2,HU Yan-hua2,ZHOU An-pei1,LI Tong-jian1,JIN Hong-guang1,HAN Xing-jie1,WEN Feng1 |
1 Jiu Jiang University, Jiujiang 332000, China 2 Dafeng Marine Industrial Institute, Nanjing Tech University, Yancheng 224100, China |
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Abstract Objective: To provide technical support for the conservation, development and utilization of Pinellia ternata (Thunb.)Breit. (P. ternata) germplasm resources by studying the propagation and preservation methods of P. ternata. Methods: Firstly, the propagation conditions were explored by screening sterilization conditions, callus induction and cluster buds induction. Secondly, the suitable conditions for germplasm preservation were explored by setting different basic media, sucrose concentration, temperature and light intensities. Finally, the methods of awakening dormant tubers and reinducing clumping buds were explored to provide a basis for the re-propagation of germplasm resources. Results: The petiole explants were sterilized with mercuric chloride for 12 minutes and inoculated into the medium supplemented with 2,4-D to induce loose callus. The explants were inoculated to MS+2.0 mg/L 6-BA+0.1 mg/L NAA+ 30 g/L sucrose + 6.0 g/L agar, pH 5.8 for cluster buds induction. MS and N6 medium was the appropriate medium for germplasm preservation. The appropriate sucrose concentration is 30 g/L, and either too low or too high sucrose concentration was not conducive to germplasm preservation. It is necessary to provide sufficient temperature and light during tuber induction. Low temperature and light avoidance conditions are not conducive to germplasm preservation. It can be stored in vitro for up to 1-2 years after the sprout tumble of cluster buds. The wake-up induction of dormant tubers can be induced by cutting tubers and then inoculating them. The germination of the petiole can be directly induced by cluster buds proliferation, while the tubers can be inoculated into MS+ 6-BA 1.0 mg/L+NAA 0.2 mg/L+ sucrose 30 g/L+ agar 6.5 g/L, pH 5.8 to induce cluster buds re-differentiation. The cluster buds can be propagated or preserved in vitro. Conclusion: Through the propagation of germplasm of P. ternata, the dormant cluster tubers can be obtained by screening appropriate conditions, which can be preserved in vitro for a long time, and then awakened and induced again, providing a technical basis for the conservation and utilization of its germplasm resources.
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Received: 14 September 2022
Published: 07 December 2022
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Cite this article:
JIA Ming-liang, FANG He-fang, ZHANG Ben-hou, HU Yan-hua, ZHOU An-pei, LI Tong-jian, JIN Hong-guang, HAN Xing-jie, WEN Feng. Propagation and Preservation of Germplasm Resources in Pinellia ternata (Thunb.)Breit.. China Biotechnology, 2022, 42(11): 18-26.
URL:
https://manu60.magtech.com.cn/biotech/10.13523/j.cb.2209032 OR https://manu60.magtech.com.cn/biotech/Y2022/V42/I11/18
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