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Construction of Yeast Surface Display System and Application in Cellulose Degrading |
LIU Ming-zhu1,ZHANG Liang1,GUO Fang1,LI Chun1,2,3,FENG Xu-dong1,**() |
1 Key Laboratory of Medical Molecule Science and Pharmaceutics Engineering, Ministry of Industry and Information Technology, Institute of Biochemical Engineering, Department of Chemical Engineering, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 100081, China 2 Key Lab for Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China 3 Center for Synthetic and Systems Biology, Tsinghua University, Beijing 100084, China |
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Abstract Cellulose is a low-cost and renewable resource with abundant reserves, but it is difficult to be used because of its compact structure. Currently, the degradation of cellulose requires the cooperation of a variety of cellulases, but the high cost and the difficulty of re-use of free cellulase have limited its wide application. Yeast surface display technology can display multiple cellulase enzymes on the cell surface after being fused with the anchoring protein, so that a yeast surface display cellulase system could be constructed. This system can degrade cellulose efficiently. On the one hand, it has the advantages of surface display, such as easy recycling, good stability, simple operation, and low cost; on the other hand, it can also degrade cellulose into glucose effectively, and has the potential to produce bioethanol by metabolism. The paper summarizes the construction principles of a yeast surface display system, and divides yeast surface display systems into direct display systems and indirect display systems according to the different anchoring methods of exogenous proteins. The direct display system is that the exogenous protein is directly connected to the cell through the anchor protein. Indirect display systems are linked to cells through exogenous proteins by means of scaffolds. Factors affecting the efficiency of the display system mainly include cell type, anchor protein, scaffold, environmental factors and promoter type. The surface display system consists of GPI system, Pir system and FlO1 system according to different anchor proteins. The yeast surface display cellulase system can ferment cellulose to produce ethanol, and a lot of progress has been made so far, which provides a reference for the construction of an efficient yeast surface display cellulase system and other multi-enzyme systems. The review describes the construction principles of a yeast surface display system, summarizes the influence of major factors on the display system, and introduces the application of this technology in the degradation of cellulose. It provides guidance for the construction of high-efficiency yeast surface display of cellulase and other multi-enzyme systems.
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Received: 19 December 2021
Published: 17 June 2022
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Corresponding Authors:
Xu-dong FENG
E-mail: xd.feng@bit.edu.cn
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