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Effects of Vitreoscilla Hemoglobin Gene on Growth and Intracellular Oxidation State of Saccharomyces cerevisiae |
XUE Zhi-yong,DAI Hong-sheng,ZHANG Xian-yuan,SUN Yan-ying,HUANG Zhi-wei() |
Key Lab of Science & Technology of Eco-textile (Ministry of Education), College of Chemistry & Chemical Engineering and Biotechnology,Donghua University, Shanghai 201620, China |
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Abstract Heterologous expression of Vitreoscilla hemoglobin gene vgb in a variety of research and industrial fermentation bacteria can solve the problem of dissolved oxygen rate in high-density fermentation. Saccharomyces cerevisiae is a classic eukaryotic model and has an important application value in the fermentation industry. However, the impact of vgb in Saccharomyces cerevisiae on cell growth is unclear. In this study, a heterologous expression plasmid YEplac195-ADH1pr-vgb containing Vistreoscilla hemoglobin gene vgb was constructed with Adh1 as promoter, and transformed into Saccharomyces cerevisiae BY4741. Growth sensitivity test showed that the heterologous expression of vgb inhibited the growth of the strain in both fermentation and non fermentation carbon sources. Then, we found the accumulation of reactive oxygen species (ROS), the change of membrane permeability and lipid peroxidation in S. cerevisiae cells overexpressing vgb by DCFH-DA and PI staining and lipid peroxidation products assay. The results showed that the overexpression of vgb changed the oxidation state in cells, promoted the accumulation of reactive oxygen (ROS), and oxidative stress caused the growth inhibition of the strain.
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Received: 28 June 2021
Published: 01 December 2021
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Corresponding Authors:
Zhi-wei HUANG
E-mail: zhiweih@dhu.edu.cn
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