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Research and Strategies of Flavins-mediated Extracellular Electron Transfer |
LI Yuan-yuan,LI Yan,CAO Ying-xiu(),SONG Hao |
School of Chemical Engineering and Technology,Tianjin University,Frontier Science Center for Synthetic Biology, Key Laboratory of Systems Bioengineering (Ministry of Education),Tianjin 300072,China |
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Abstract Extracellular electron transfer is the process that electroactive microorganisms (EAMs) acquire energy from the environment by extending respiratory chains to external electron acceptors. Organism Shewanella oneidensis is widely used as a model to study emerging bioelectrochemical technologies, including microbial fuel cell (MFC), microbial electrosynthesis, as well as pollutant degradation in bioremediation. A previous study reported that almost all electrons generated by S. oneidensis were transmitted into acceptors relying on flavins, including flavin mononucleotide (FMN) and riboflavin (RF). What’s more, flavins-mediated extracellular electron transfer is the rate-limiting step in the process of electron transmission. However, flavins secreted by wild-type S. oneidensis are negligible, and the engineering modifications for S. oneidensis are limited, both of which seriously hinder the electrons transfer process, and enable the main bottleneck of restricting the electrons transmission. In this study, the regulatory factors of flavin synthesis were systematically demonstrated from the perspective of flavin biosynthesis and transcriptional regulation relying on the mechanisms of flavins-mediated electron transfer in S. oneidensis. Besides, the strategies utilizing metabolic engineering, synthetic biology and modification of electrode materials for improving flavins-mediated electron transfer in recent years were summarized. Further, it can be proposed that in the future, systematic research and expression tools can be utilized to accelerate the flavins-mediated extracellular electron transfer in EAMs.
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Received: 21 April 2021
Published: 08 November 2021
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Corresponding Authors:
Ying-xiu CAO
E-mail: caoyingxiu@tju.edu.cn
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