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Preparation and Drug Release Properties of Keratin-loaded Nanoparticles |
LI Jia-xin1,ZHANG Zheng1,LIU He1,YANG Qing1,LV Cheng-zhi2,**(),YANG Jun1,**() |
1 School of Bioengineering, Dalian University of Technology, Dalian 116024,China 2 Dalian Dermatosis Hospital,Dalian 116021,China |
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Abstract Objective: Keratin was used as the drug carrier material, and the hydrophilic drug rhodamine and hydrophobic drug curcumin were loaded to prepare the intelligent responsive drug delivery system, and the pH and redox responsiveness of drug release were studied. Methods: The exopolysaccharide of Lactobacillus plantarum was introduced into the reaction system of sodium selenite and ascorbic acid, and KNP was synthesized at room temperature. The size, morphology, structure and stability of KNP were studied by tungsten filament scanning electron microscopy (SEM), dynamic light scattering (DLS), Fourier transform infrared spectroscopy (FTIR) and drug release experiments in vitro. Results:KNP with uniform particle size of about 300 nm was successfully prepared, which can load hydrophilic and hydrophobic drugs. Keratin based nanoparticles exhibited pH and glutathione (GSH) dual responsive release characters. In addition, these drug-loaded particles showed potent prolongation duration, good stability, sustainable and controllable release than the original drug. Conclusion: KNP with high stability, good water dispersion and responsiveness can be prepared simply and safely, and the drug delivery system of keratin is an appropriate candidate drug carrier.
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Received: 30 April 2021
Published: 31 August 2021
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Corresponding Authors:
Cheng-zhi LV,Jun YANG
E-mail: dlpfb126.com;junyang@dlut.edu.cn
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