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Research Progress of Key Enzymes in Terpene Biosynthesis |
MIAO Yi-nan1,LI Jing-zhi1,WANG Shuai1,LI Chun1,2,WANG Ying1,**() |
1 Key Laboratory of Medical Molecule Science and Pharmaceutics Engineering, Ministry of Industry and Information Technology, Institute of Biochemical Engineering, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 102488, China 2 Department of Chemical Engineering, Tsinghua University, Beijing 100084, China |
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Abstract Terpenes are a large class of highly diverse natural products with various physiological activities such as anti-tumor, anti-oxidation and immune regulation. Therefore, they are widely used in the fields of medicine and health, food, cosmetics, and biofuels. However, obtaining terpene compounds directly from natural resources is low efficient, costly, and often has an adverse impact on the ecological environment, making it impossible to achieve green and sustainable production. Microbial synthesis of terpenes has attracted much attention in recent years. Researchers have conducted explorations from the construction and regulation of synthetic pathways, protein engineering, and fermentation process optimization, and have obtained fruitful results. Among them, the efficiency of key enzymes in the synthetic pathway plays an important role in the microbial production of terpenes. Research on key enzymes is of great significance for improving the ability of microorganisms to synthesize terpenes and thus accelerating the large-scale application of microbial production of such natural products. Here, four key enzymes in the synthetic pathway of terpenes including 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR), 1-deoxy-D-xylulose-5-phosphate synthase (DXS), isoprenyl diphosphate synthase (IDS), and terpene synthase (TPS) were introduced. The regulation of catalytic activities of key enzymes via metabolic engineering, protein engineering and synthetic biology to improve the efficiency of microbial synthesis of terpenes as well as the prospects of using microorganisms to synthesize terpenes were also reviewed.
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Received: 24 February 2021
Published: 06 July 2021
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Corresponding Authors:
Ying WANG
E-mail: wy2015@bit.edu.cn
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