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Knockdown of Deubiquitinase USP13 Inhibits the Proliferation of K562 Cells |
TAO Shou-song1,REN Guang-ming2,YIN Rong-hua2,YANG Xiao-ming1,2,MA Wen-bing3,**(),GE Zhi-qiang1,**() |
1 Department of Pharmaceutical Engineering, Tianjin University, Tianjin 300072, China 2 Beijing Institute of Lifeomics, Academy of Military Medical Sciences, Academy of Military Sciences, Beijing 100850, China 3 Institute of Health Service and Blood Academy of Military Medical Sciences, Beijing 100850, China |
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Abstract Objective: Study the effect of deubiquitinating enzyme USP13 on the proliferation and apoptosis of human chronic myeloid leukemia K562 cells, and explore the underlying mechanism. Methods: Construction of the pLKO.1-shUSP13-GFP lentiviral interference vector and establishment of the USP13 knockdown K562 cell line using lentivirus. Western blot detected the USP13 knockdown efficiency in K562 cells. Flow cytometry analyzed the effect of USP13 knockdown on the proliferation and apoptosis of K562 cells. Co-immunoprecipitation and protein ubiquitination experiments explored the regulation mechanism of USP13 on K562 cells. Results: The pLKO.1-shUSP13-GFP vector was successfully constructed, and K562 cell line with stable knockdown of USP13 was obtained using the lentiviral system. Flow cytometry results showed that knocking down USP13 promoted K562 cell apoptosis and inhibited cell proliferation. Molecular mechanism studies found that knockdown of USP13 decreases c-Myc level by enhancing its ubiquitination. Conclusions: Data preliminarily revealed the molecular mechanism of USP13 regulating the proliferation and apoptosis of K562 cells, providing a potential target for the treatment of chronic myeloid leukemia.
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Received: 27 January 2021
Published: 01 June 2021
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Corresponding Authors:
Wen-bing MA,Zhi-qiang GE
E-mail: mawenbing003@163.com;gezhiq@tju.edu.cn
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