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| The Structural Characteristics and Engineering Reconstruction of Flavin-dependent Halogenase |
WANG Yi-han,LI Hai-yan,XUE Yong-chang( ) |
| School of Biological Engineering, Dalian Polytechnic University, Dalian 116034, China |
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Abstract Halides are a kind of compounds which catalyzed by halogenase to the addition of halogen in secondary metabolites, have unique physiological and biochemical functions. Flavin-dependent halogenases have good regional selectivity and stability, and similar FAD binding sites with slight difference in substrates made them very important in industrial application. Therefore, the studies on its structure and synthetic pathway as well as random mutagenesis and directed modification of protein engineering are very important for its industry application. In this paper, the structural characteristics and engineering modification of flavin-dependent halogenases with high regional selectivity are reviewed, and it is of guiding significance for the application of engineering-modification flavin-dependent halides in industrial production.
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Received: 17 December 2020
Published: 30 April 2021
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Corresponding Authors:
Yong-chang XUE
E-mail: xueych@dlpu.edu.cn
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