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中国生物工程杂志

China Biotechnology
China Biotechnology
China Biotechnology  2021, Vol. 41 Issue (2/3): 7-13    DOI: 10.13523/j.cb.2009002
    
Long Non-coding RNA Expression Profile in A549 Cells Infected with Human Respiratory Syncytial Virus
LIU Mei-qin,GAO Bo,JIAO Yue-ying,LI Wei,YU Jie-mei,PENG Xiang-lei,ZHENG Yan-peng,FU Yuan-hui,HE Jin-sheng()
College of Life Sciences & Bioengineering, Beijing Jiaotong University, Beijing 100044, China
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Abstract  

Objective: To better understand the possible molecular mechanism of the interaction between the host and human respiratory syncytial virus (RSV), the long non-coding RNA expression profile in A549 cells infected by RSV was investigated. Methods: After 24 hours of A549 cells infected with 1 MOI RSV and extracted for RNA samples, lncRNA differential expression profile was screened by Agilent’s lncRNA expression profile chip and compared with the A549 cell control. LncRNA differential expression profile was verified by real-time quantitative PCR (qRT-PCR). Bioinformatics analysis of differential mRNA and lncRNA was performed by GO and KEGG. Results: A total of 1 463 lncRNA and 1 552 mRNAs were significantly up-regulated in RSV-infected A549 cells compared with A549 cell control, while 944 lncRNA and 1 489 mRNAs were down-regulated. LncRNA and the predicted target genes were mainly enriched in the immune response. The expression profile of the selected lncRNA was confirmed by qRT-PCR. Conclusion: LncRNA expression profile changes significantly after RSV infection of A549 cells, but the underlining mechanism in the interaction between RSV and host needs to be further explored.

Key words： Human respiratory syncytial virus      A549 cells      Long non-coding RNA      Expression profile     
Received: 01 September 2020      Published: 08 April 2021
ZTFLH:  Q812  
Corresponding Authors: Jin-sheng HE     E-mail: jshhe@bjtu.edu.cn
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Mei-qin LIU
Bo GAO
Yue-ying JIAO
Wei LI
Jie-mei YU
Xiang-lei PENG
Yan-peng ZHENG
Yuan-hui FU
Jin-sheng HE
Cite this article:

LIU Mei-qin,GAO Bo,JIAO Yue-ying,LI Wei,YU Jie-mei,PENG Xiang-lei,ZHENG Yan-peng,FU Yuan-hui,HE Jin-sheng. Long Non-coding RNA Expression Profile in A549 Cells Infected with Human Respiratory Syncytial Virus. China Biotechnology, 2021, 41(2/3): 7-13.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.2009002     OR     https://manu60.magtech.com.cn/biotech/Y2021/V41/I2/3/7

RSV感染的A549细胞中上调的lncRNAs RSV感染的A549细胞中下调的lncRNAs
lncRNA ID FC P value lncRNA ID FC P value
ENST000007613.1 168.389 0.000 594 ENST00000520156.1 22.824 0.000 992
RNA147145|p0249_imsncRNA392 117.749 0.000 083 ENST00000579368.1 13.528 0.000 106
ENST00000415810.1 92.435 0.000 213 uc010lcl.3 11.711 0.000 133
HIT000071394 85.878 0.000 213 ENST00000560973.1 10.848 0.006 189
uc003ylb.1 82.100 0.000 424 ENST00000560237.1 9.344 0.000 017
TCONS_00027056 71.146 0.000 215 ENST00000599209.1 8.865 0.000 839
XR_426856.1 70.406 0.000 122 XR_429553.1 8.307 0.000 789
ENST00000600152.1 66.528 0.000 038 TCONS_00028233 8.162 0.005 519
ENST00000592728.1 65.052 0.000 355 ENST00000521373.1 7.929 0.003 902
ENST00000595705.1 59.927 0.001 780 XR_247160.1 7.482 0.000 099
Table 1 Compared with uninfected A549 cells, the 10 most significantly up-regulated and down-regulated lncRNAs in RSV-infected A549 cells
RSV感染的A549细胞中上调的mRNAs RSV感染的A549细胞中下调的mRNAs
Genbank Accession FC P value Genbank Accession FC P value
NM_172139 4 903.417 0.000 011 NM_001005190 34.342 0.000 026
NM_001001437 1 914.344 0.000 012 NM_014405 14.089 0.007 145
NM_172140 1 675.346 0.000 076 NM_207362 13.127 0.000 356
NM_005409 1 025.258 0.000 089 NM_001001925 11.840 0.000 955
NM_017523 469.671 0.000 023 NM_001145064 10.694 0.000 069
NM_001565 457.013 0.000 045 NM_004975 10.657 0.000 007
NM_001565 374.110 0.000 019 NM_021871 10.468 0.002 490
NM_080657 330.406 0.000 049 NM_001736 10.147 0.006 427
NM_017523 288.632 0.000 007 NM_032294 9.232 0.000 058
XM_006724857 278.801 0.000 170 NM_014376 9.077 0.000 063
Table 2 Compared with uninfected A549 cells, the 10 most significantly up-regulated and down-regulated mRNAs in RSV-infected A549 cells
Fig.1 Cluster analysis of differentially expressed lncRNA and mRNA Compared with the control group, there are distinguishable hierarchical clustering of lncRNA (a) and mRNA (b) expression profiles in A549 cells infected with RSV. Higher and lower expression levels are represented as red and green, respectively
Fig.2 GO and pathway enrichment analysis results of mRNA regulated by IncRNA Compared with the control group, the GO enrichment analysis (a) and KEGG pathway analysis (b) of the differentially expressed mRNA regulated by lncRNA in A549 cells infected with RSV
Fig.3 qRT-PCR results of 18 significantly differentially expressed lncRNAs
Fig. 4 The results of qRT-PCR verification of 5 significantly differentially expressed mRNAs
Fig.5 The effect of knocking down lncRNA on the protein expression level of A549 cells (a) The effect of knocking down P3377 on the expression level of OAS2 in A549 cells (b) The effect of knocking down P6810 on the expression level of BST2 in A549 cells
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