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Synthesis and Regulation of Poly-γ-glutamic Acid with Different D/L Monomer Ratios |
ZHU Ya-xin1,2,DUAN Yan-ting1,2,GAO Yu-hao1,2,WANG Ji-yue1,2,ZHANG Xiao-mei3,ZHANG Xiao-juan1,2,XU Guo-qiang1,2,**(),SHI Jin-song3,XU Zheng-hong1,2 |
1 Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China 2 National Engineering Laboratory for Food Fermentation Technology and Technology, Jiangnan University, Wuxi 214122, China 3 School of Pharmaceutics, Jiangnan University, Wuxi 214122, China |
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Abstract Poly-γ-glutamic acid (γ-PGA) is a kind of poly-amino acid formed by polymerization of L-glutamic acid (L-Glu) and / or D-glutamic acid (D-Glu), and is widely used in cosmetics, medicine and other fields. The stereochemical composition of its monomer often affects the properties and applications of the product. Therefore, it is of great significance to control the monomer ratio of D-Glu/L-Glu (D/L monomer ratio) in γ-PGA. In our previous study, Corynebacterium glutamicum was used as the chassis to overexpress γ-PGA synthetase from Bacillus licheniformis, and synthesize γ-PGA with L-Glu as the main component. On this basis, different concentrations of D-Glu were added exogenously to synthesize γ-PGA with D-Glu accounting for 15.71% ~ 33.52%. Then, the glutamate racemase from Bacillus subtilis was overexpressed in the recombinant bacteria, and three RBS of different strengths were used to regulate its expression level, however, and γ-PGA with D-Glu accounting for a narrow range (30.82%~34.59%) was synthesized. Subsequently, four different strength promoters were used to regulate the expression level of glutamate racemase, and γ-PGA with D-Glu accounting for 32.71%~52.53% was synthesized. A rational strategy for regulating the D/L monomer ratio of γ-PGA was proposed, and γ-PGA with a D-Glu ratio of 2.90%~52.53% was synthesized, which laid the foundation for the efficient synthesis of γ-PGA with different D/L monomer ratios.
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Received: 17 September 2020
Published: 09 February 2021
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Corresponding Authors:
Guo-qiang XU
E-mail: xuguoqiang@jiangnan.edu.cn
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Cite this article:
ZHU Ya-xin, DUAN Yan-ting, GAO Yu-hao, WANG Ji-yue, ZHANG Xiao-mei, ZHANG Xiao-juan, XU Guo-qiang, SHI Jin-song, XU Zheng-hong. Synthesis and Regulation of Poly-γ-glutamic Acid with Different D/L Monomer Ratios. China Biotechnology, 2021, 41(1): 1-11.
URL:
https://manu60.magtech.com.cn/biotech/10.13523/j.cb.2009029 OR https://manu60.magtech.com.cn/biotech/Y2021/V41/I1/1
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