Please wait a minute...

中国生物工程杂志

China Biotechnology
China Biotechnology
China Biotechnology  2020, Vol. 40 Issue (11): 90-95    DOI: 10.13523/j.cb.2007038
    
Research Progress on Separation and Purification of Mannan Oligosaccharide
PAN Bing-jv,ZHANG Wan-yi,SHEN Hui-tao,LIU Ting-ting,LI Zhong-yuan,LUO Xue-gang,SONG Ya-jian()
College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China
Download: HTML   PDF(395KB) HTML
Export: BibTeX | EndNote (RIS)      

Abstract  

Mannan oligosaccharides have a series of physiological functions such as, lower blood fat, resist colon inflammation, enhance immunity and regulate intestinal flora and have broad application prospects in the fields of food and medicine. The mannan-oligosaccharide products prepared by the hydrolysis method usually contain monosaccharides, unhydrolyzed polysaccharides, oligosaccharides with different polymerization degree, salt ions and other impurities, which need further separation and purification. The main purification methods of mannose oligosaccharides in recent years are reviewed, including column chromatography, membrane separation, ethanol precipitation and microbial fermentation. The principle, application scope and application examples of various methods are summarized, and the advantages and disadvantages of different methods are analyzed.

Key words： Mannan oligosaccharide      Separation      Purification     
Received: 21 July 2020      Published: 11 December 2020
ZTFLH:  Q-1  
Corresponding Authors: Ya-jian SONG     E-mail: songyajian@tust.edu.cn
Service
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
RSS
Articles by authors
Bing-jv PAN
Wan-yi ZHANG
Hui-tao SHEN
Ting-ting LIU
Zhong-yuan LI
Xue-gang LUO
Ya-jian SONG
Cite this article:

PAN Bing-jv,ZHANG Wan-yi,SHEN Hui-tao,LIU Ting-ting,LI Zhong-yuan,LUO Xue-gang,SONG Ya-jian. Research Progress on Separation and Purification of Mannan Oligosaccharide. China Biotechnology, 2020, 40(11): 90-95.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.2007038     OR     https://manu60.magtech.com.cn/biotech/Y2020/V40/I11/90

分离方法 柱层析法
硅胶柱 活性炭柱 离子交换柱 凝胶柱
优点 除去单糖及多糖组分,提高甘露寡糖浓度;适于工业大量生产 分离得到单一聚合度寡糖;适于工业大量生产 除去单糖及多糖组分,提高甘露寡糖浓度;脱盐脱色;适于工业大量生产 分离得到一定聚合度的寡糖、纯度较高的单一聚合度寡糖
缺点 不能分离得到单一聚合度寡糖 制备的寡糖纯度低;样品复杂时分离效果差 不能分离得到单一聚合度寡糖 生产成本高;大量制备困难,不适于工业大量生产
Table 1 Advantages and disadvantages of different separation methods
[1]   Suwannaporn P, Tester R F, Al-Ghazzewi F H, et al. Effect of short term administration of konjac glucomannan hydrolysates on adult blood lipid parameters and glucose concentrations. Nutrition & Food Science, 2015,45(4):616-624.
[2]   Hoving L R, Katiraei S, Heijink M, et al. Dietary mannan oligosaccharides modulate gut microbiota, increase fecal bile acid excretion, and decrease plasma cholesterol and atherosclerosis development. Mol Nutr Food Res, 2018,62(10):e1700942.
doi: 10.1002/mnfr.201700942 pmid: 29665623
[3]   Sohail M U, Ijaz A, Yousaf M S, et al. Alleviation of cyclic heat stress in broilers by dietary supplementation of mannan-oligosaccharide and Lactobacillus-based probiotic: dynamics of cortisol, thyroid hormones, cholesterol, C-reactive protein, and humoral immunity. Poult Sci, 2010,89(9):1934-1938.
doi: 10.3382/ps.2010-00751 pmid: 20709978
[4]   武书庚. 甘露低聚糖可替代抗生素. 国外畜牧科技, 2000,27(1):19-20.
[4]   Wu S G. Mannan oligosaccharides can replace antibiotics. Animal Husbandry Technology Abroad, 2000,27(1):19-20.
[5]   Liu X Y, Chen S, Yan Q J, et al. Effect of Konjac mannan oligosaccharides on diphenoxylate induced constipation in mice. Journal of Functional Foods, 2019,57:399-407.
[6]   Wang C H, Lai P, Chen M E, et al. Antioxidative capacity produced by Bifidobacterium-and Lactobacillus acidophilus-mediated fermentations of konjac glucomannan and glucomannan oligosaccharides. J Sci Food Agric, 2008,88(7):1294-1300.
[7]   Liu R, Li Y, Zhang B. The effects of konjac oligosaccharide on TNBS-induced colitis in rats. Int Immunopharmacol, 2016,40(40):385-391.
[8]   赵若春, 赵晓勤, 毛开云. 我国功能性低聚糖产业发展现状及发展趋势分析. 生物产业技术, 2018, (6):5-8.
[8]   Zhao R C, Zhao X Q, Mao K Y. Analysis of development status and development trend of functional oligosaccharide industry in my country. Biotechnology, 2018, (6):5-8.
[9]   Yang J K, Chen Q C, Zhou B, et al. Manno-oligosaccharide preparation by the hydrolysis of konjac flour with a thermostable endo-mannanase from Talaromyces cellulolyticus. Journal of Applied Microbiology, 2019,127(2):520-532.
doi: 10.1111/jam.14327 pmid: 31099168
[10]   Pradeep G C, Seung S C, Yun H C, et al. An extremely alkaline mannanase from Streptomyces sp. CS428 hydrolyzes galactomannan producing series of mannooligosaccharides. World Journal of Microbiology and Biotechnology, 2016,32(5):84.
[11]   Nguy Q A, Cho E J, Enlee D S, et al. Development of an advanced integrative process to create valuable biosugars including manno-oligosaccharides and mannose from spent coffee grounds. Bioresource Technology, 2019,272(272):209-216.
[12]   Srivastava P K, Panwar D, Prashanth K V H, et al. Structural characterization and in vitro fermentation of β-mannooligosaccharides produced from locust bean gum by GH-26 endo-β-1,4-mannanase (ManB-1601). Journal of Agricultural & Food Chemistry, 2017,65(13):2827-2838.
doi: 10.1021/acs.jafc.7b00123 pmid: 28225615
[13]   王苗, 蒋敏, 李恒, 等. 乳酸菌对魔芋甘露低聚糖的降解与利用. 食品与发酵工业, 2016,42(11):20-24.
[13]   Wang M, Jiang M, Li H, et al. Degradation and utilization of konjac oligosaccharides by lactic acid bacteria. Food and Fermentation Industry, 2016,42(11):20-24.
[14]   许牡丹, 汤木红, 王小燕. 硅胶柱层析法分离纯化甘露低聚糖. 食品工业科技, 2008,29(9):188-190.
[14]   Xu M D, Tang M H, Wang X Y. Separation and purification of mannan oligosaccharides by silica gel column chromatography. Food Industry Technology, 2008,29(9):188-190.
[15]   王绍云. 魔芋葡甘露寡糖的酶解制备及其分离纯化的研究. 北京: 中国农业科学院, 2009.
[15]   Wang S Y. Study on enzymolysis preparation and separation and purification of konjac glucomannan oligosacce. Beijing: Chinese Academy of Agricultural Sciences, 2009.
[16]   陈秀敏, 吕秀菊, 傅德贤, 等. 魔芋葡甘露寡糖的制备、化学结构及对胰岛NO自由基释放量的影响. 中国生物化学与分子生物学报, 2002,18(6):89-93.
[16]   Chen X M, Lv X J, Fu D X, et al. Preparation, chemical structure of konjac glucomannan oligosaccharide and its effect on islet NO release. Chinese Journal of Biochemistry and Molecular Biology, 2002,18(6):89-93.
[17]   吴东儒. 糖类的生物化学. 上海: 高等教育出版社, 1987: 884-885.
[17]   Wu D R. Sugar biochemistry. Shanghai: Higher Education Press, 1987: 884-885.
[18]   冯咏梅, 常秀莲, 王文华, 等. 离子交换层析快速高效分离低聚糖. 食品与发酵工业, 2009,35(3):179-182.
[18]   Feng Y M, Chang X L, Wang W H, et al. Ion exchange chromatography for fast and efficient separation of oligosaccharides. Food and Fermentation Industry, 2009,35(3):179-182.
[19]   杨伟东. 离子交换树脂分离纯化葡甘露低聚糖工艺研究. 食品与发酵科技, 2019,55(4):14-17.
[19]   Yang W D. Study on separation and purification of glucomannan oligosaccharide by cation exchange resin. Food and fermentation technology, 2019,55(4):14-17.
[20]   许牡丹, 姚甲玉, 毛跟年, 等. 离子交换法分离纯化甘露低聚糖. 食品科技, 2008, (8):150-152.
[20]   Xu M D, Yao J Y, Mao G N, et al. Separation and purification of mannan oligosaccharides by ion exchange. Food Technology, 2008, (8):150-152.
[21]   徐振林, 杨幼慧, 孙远明, 等. 辐照魔芋葡甘露聚糖的应用研究. 中国食品学报, 2008,8(1):78-82.
[21]   Xu Z L, Yang Y H, Sun Y M, et al. Study on application of irradiated konjac glucomannan. Chinese Journal of Food, 2008,8(1):78-82.
[22]   Nopvichai C, Charoenwongpailboon T, Luengluepunyan T, et al. Production and purification of mannan oligosaccharide with epithelial tight junction enhancing activity. PEERJ, 2019,7(7):e7206.
[23]   Ghosh A, Verma K, Tingirikari J R, et al. Recovery and purification of oligosaccharides from copra meal by recombinant endo-β-mannanase and deciphering molecular mechanism involved and its role as potent therapeutic agent. Molecular Biotechnology, 2015,57(2):111-127.
[24]   Srivastava P K, Kapoor M. Cost-effective endo-mannanase from Bacillus sp. CFR1601 and its application in generation of oligosaccharides from guar gum and as detergent additive. Prep Biochem Biotechnol, 2014,44(4):392-417.
doi: 10.1080/10826068.2013.833108 pmid: 24320239
[25]   Jian H L, Zhu L W, Zhang W M, et al. Enzymatic production and characterization of manno-oligosaccharides from gleditsia sinensis galactomannan gum. Int J Biol Macromol, 2013,55(55):282-288.
[26]   尧咏. 魔芋低聚糖的分离纯化、结构初探及免疫活性研究. 武汉: 华中农业大学, 2016.
[26]   Xiao Y. Study on separation, purification, structure and immune activity of konjac oligosaccharide. Wuhan: Huazhong Agricultural University, 2016.
[27]   Saska M. Member based separations andcounter current multicolumn systems for decolorizarion, ion exchange and ion exclusion:emerging technologies for the sugar industry. Indian Sugar, 1997, ( 3):169-183.
[28]   Jian W J, Sun Y M, Huang H, et al. Study on preparation and separation of konjac oligosaccharides. Elsevier, 2013,92(2):1218-1224.
[29]   史劲松, 郭鸿飞, 孙达峰, 等. 半乳甘露寡糖的膜法精制工艺研究. 中国野生植物资源, 2009,28(4):45-47.
[29]   Shi J S, Guo H F, Sun D F, et al. Study on the membrane refining process of galactomannan oligosaccharide. Chinese Wild Plant Resources, 2009,28(4):45-47.
[30]   郭跃平, 徐广伟, 韦何雯, 等. 利用咖啡豆渣酶法制备甘露低聚糖的研究. 食品研究与开发, 2017,38(21):59-64.
[30]   Guo Y P, Xun G W, Wei H W, et al. Study on the preparation of mannan oligosaccharides by enzymatic method of coffee bean residue. Food Research and Development, 2017,38(21):59-64.
[31]   方积年, 丁侃. 天然药物:多糖的主要生物活性及分离纯化方法. 中国天然药物, 2007,5(5):338-347.
[31]   Fang J N, Ding K. Natural medicine:The main biological activity and separation and purification methods of polysaccharides. Chinese Natural Medicine, 2007,5(5):338-347.
[32]   李延啸. 米黑根毛霉和微孢根霉β-甘露聚糖酶的分子改造、高效表达及应用. 北京: 中国农业大学, 2018.
[32]   Li Y X. Molecular modification, high expression and application of Rhizopus oryzae and Rhizopus oryzae β-mannanase. Beijing: China Agricultural University, 2018.
[33]   Liu J H, Xu Q H, Zhang J J, et al. Preparation, composition analysis and antioxidant activities of konjac oligo-glucomannan. Carbohydrate Polymers, 2015,130:398-404.
doi: 10.1016/j.carbpol.2015.05.025 pmid: 26076641
[34]   谢瑾. 微生物发酵法提高水苏糖纯度的研究. 广州: 华南理工大学, 2018.
[34]   Xe J. Study on improving the purity of stachyose by microbial fermentation.South China University of Technology, Guangzhou: 2018.
[35]   吴长菲, 董岩岩, 李俊俊, 等. 魔芋葡甘露低聚糖的酶法制备工艺的初步研究. 生物技术通报, 2010, (1):118-122.
[35]   Wu C F, D Y Y, Li J J, et al. Preliminary study on enzymatic preparation technology of konjac glucomannan oligosaccharide. Biotechnology Bulletin, 2010, (1):118-122.
[36]   张琳. 魔芋低聚糖的制备及其性质研究. 吉林: 吉林大学, 2013.
[36]   Zang L. Study on preparation and properties of konjac oligosaccharide. Jilin: Jilin University, 2013.
[1] ZHANG Ling,CAO Xiao-dan,YANG Hai-xu,LI Wen-lei. The Application of Continuous Purification in Affinity Chromatography and Evaluation of Production Scale-up[J]. China Biotechnology, 2021, 41(6): 38-44.
[2] LV Yi-fan,LI Geng-dong,XUE Nan,LV Guo-liang,SHI Shao-hui,WANG Chun-sheng. Prokaryotic Expression, Purification of LbCpf1 Protein Gene and in Vitro Cleavage Activity Assay[J]. China Biotechnology, 2020, 40(8): 41-48.
[3] JIANG Dan-dan,WANG Yun-long,LI Yu-lin,Zhang Yi-qing. Study on the Delivery of RGD Modified Virus-Like Particles to ICG Targeted Tumors[J]. China Biotechnology, 2020, 40(7): 22-29.
[4] XIE Hang-hang,BAI Hong-mei,YE Chao,CHEN Yong-jun,YUAN Ming-cui,MA Yan-bing. The Purification Procedure for the Recombinant HBcAg Virus-like Particle Easy to Generate Aggregation[J]. China Biotechnology, 2020, 40(5): 40-47.
[5] WEI Wei,CHANG Bao-gen,WANG Ying,LU Fu-ping,LIU Fu-feng. Heterologous Expression, Purification and Aggregation Characterization of Tau Core Fragment 306-378[J]. China Biotechnology, 2020, 40(5): 22-29.
[6] LIU Zhen-zhen,TIAN Da-yong. Development of Sucrose Density Gradient Centrifugation Purification Process for Rabies Vaccine[J]. China Biotechnology, 2020, 40(4): 25-33.
[7] ZHU Tong-tong,YANG Lei,LIU Ying-bao,SUN Wen-xiu,ZHANG Xiu-guo. Purification and Crystallization of PcCRN20-C from Phytophthora capsici[J]. China Biotechnology, 2020, 40(1-2): 116-123.
[8] Jing-yun FENG,Ling-qia SU,Jing WU. Synthesis and Extraction of Trehalose from Multiple Enzymes Reaction[J]. China Biotechnology, 2019, 39(7): 65-70.
[9] Yu-feng XIE,Xue-mei HAN,Fu-ping LU. Expression, Purification and Enzymatic Properties of β-glucosidase from Lactobacillus paracasei[J]. China Biotechnology, 2019, 39(5): 72-79.
[10] JING Jia-mei,XUN Xin,WANG Min,PENG Ru-chao,SHI Yi. Expression and Purification of C-terminal of Arenavirus Polymerase and Screening of Crystallization Conditions[J]. China Biotechnology, 2019, 39(12): 18-23.
[11] ZHU Meng-lu,WANG Xue-yu,LIU Xin,LU Fu-ping,SUN Deng-yue,QIN Hui-min. Heterologous Expression, Purification and Enzymatic Properties of a Novel Leucine 5-Hydroxylase[J]. China Biotechnology, 2019, 39(12): 24-34.
[12] Chao-di TONG,Jian-ping WU,Li-rong YANG,Gang XU. Crystal Structural Analysis of DehDIV-R by X-ray Crystallography[J]. China Biotechnology, 2018, 38(8): 19-25.
[13] Jun-jun CHEN,Ying LOU,Yuan-xing ZHANG,Qin LIU,Xiao-hong LIU. Expression and Purification of Proliferating Cell Nuclear Antigen in Spodoptera frugiperda Cells[J]. China Biotechnology, 2018, 38(7): 14-20.
[14] Shi-jie LI,Yan-kun YANG,Meng LIU,Zhong-hu BAI,Jian JIN. Efficient Expression of SUMO Protease Ulp1 and Used to Express and Purified scFv by His-SUMO tag[J]. China Biotechnology, 2018, 38(3): 51-61.
[15] Yuan-qiao CHEN,Ding-pei LONG,Xiao-xue DOU,Run QI,Ai-chun ZHAO. Studies on the Protein Purification Ability of an ELP30-Tag in Prokaryotic Expression System[J]. China Biotechnology, 2018, 38(2): 54-60.
主管：中国科学院 　主办：中国科学院文献情报中心 　中国生物技术发展中心 　中国生物工程学会