Advances in Detection and Typing of Diarrheal <i>Escherichia coli</i> with PCR

doi:10.13523/j.cb.2001028

China Biotechnology

2020, Vol. 40

Issue (7): 82-90 DOI: 10.13523/j.cb.2001028

Advances in Detection and Typing of Diarrheal Escherichia coli with PCR

HUANG Zhao-hong¹,HUANG Yun-hong¹,HUANG Yan-mei²,LONG Zhong-er¹,SHAN Shan^1,^**(

)

1 College of Life Sciences, Nanchang Key Laboratory of Microbial Resources Exploitation & Utilization from Poyang Lake Wetland, Jiangxi Normal University, Nanchang 330022, China
2 Jiangxi YeLi Medical Device Co. Ltd., Nanchang 330008, China

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Abstract

Diarrheal Escherichia coli is a kind of foodborne pathogens that can cause human diarrhea disease. It will be effectively to narrow the scope of the outbreak disease if the source of diarrheal Escherichia coli could be confirmed at the first time. The establishment of a simple and efficient method for the detection and typing of diarrheal Escherichia coli is the key for food safety and epidemic control. The methods based on molecular biology technology were constantly standardized and normalized in order to satisfy the need of rapid detection and typing of diarrheal Escherichia coli.The recent advances in detection and typing of diarrheal Escherichia coli by molecular biological technique was reviewed. The principle, advantages and disadvantages of multiplex polymerase chain reaction, quantitative real-time PCR and isothermal amplification of nucleic acid were introduced in detail. It will provide reference for the selection of traceability methods for pathogenic bacteria, and have the great significance for the prevention and control of epidemic spread caused by pathogenic bacteria.

Key words： Diarrheal Escherichia coli PCR technique Detection Typing

Received: 12 January 2020 Published: 13 August 2020

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Corresponding Authors: Shan SHAN E-mail: ncuskshanshan@163.com

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Cite this article:

HUANG Zhao-hong,HUANG Yun-hong,HUANG Yan-mei,LONG Zhong-er,SHAN Shan. Advances in Detection and Typing of Diarrheal Escherichia coli with PCR. China Biotechnology, 2020, 40(7): 82-90.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.2001028 OR https://manu60.magtech.com.cn/biotech/Y2020/V40/I7/82

Table 1 Different techniques to detection and typing for DEC and their advantages or disadvantages


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