Advances in <i>Lactic Acid Bacteria</i> Gene Modification

doi:10.13523/j.cb.2001017

China Biotechnology

2020, Vol. 40

Issue (6): 84-92 DOI: 10.13523/j.cb.2001017

Advances in Lactic Acid Bacteria Gene Modification

FAN Bin^1,²,CHEN Huan^1,²,SONG Wan-ying^1,²,CHEN Guang^1,²,WANG Gang^1,^2,^3,^**(

)

1 Jilin Agricultural University, College of Life Science, Changchun 130118,China
2 Key Laboratory of Straw Biology and Utilization, the Ministry of Education,Jilin Agricultural University,Changchun 130118,China
3 Cofco Biochemical Co,LTD of Jilin,Changchun 130033,China

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Abstract

Lactic acid bacteria (LAB) is a Gram-positive, obligate anaerobic bacterium, and the main production of lactic acid. It was one of the most important industry strain which was used in food industry, pharmaceutical industry and chemicals industry. In recent years, with the continuous improvement of the industry application of LAB, studies were focused on physiological and biochemical characteristics, acid tolerance characteristic, metabolic pathways and mechanism of lactic acid production of lactic acid bacteria. So, it is necessary to establish a stable and efficient gene-modifying method for analyzing the functions of key genes and geneme network and adjusting the metabolic pathway. The paper reviews the recent progress and future prospects of LAB gene-modifying technologies.

Key words： Lactic acid bacteria Gene modification Homologous recombination Loci specific Linear DNA CRISPR

Received: 05 January 2020 Published: 23 June 2020

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Corresponding Authors: Gang WANG E-mail: gangziccc@163.com

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Cite this article:

FAN Bin,CHEN Huan,SONG Wan-ying,CHEN Guang,WANG Gang. Advances in Lactic Acid Bacteria Gene Modification. China Biotechnology, 2020, 40(6): 84-92.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.2001017 OR https://manu60.magtech.com.cn/biotech/Y2020/V40/I6/84

Fig.1 Single exchange homologous recombination

Fig.2 Double exchange homologous recombination for gene knockout

Fig.3 Double exchange homologous recombination for gene integration

Fig.4 Site-specific recombination

Fig.5 Cre/lox P gene editing based on lox66 and lox71

Fig 6 Single and double chain recombination

Fig 7 CRISPER/Cas9 gene editing


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