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| Recombinant Saccharomyces cerevisiae Expressing Helicobacter pylori VacA Protein and Its Immunogenicity Analysis |
CEN Qian-hong,GAO Tong,REN Yi,LEI Han( ) |
| College of Medicine, Southwest Jiaotong University, Chengdu 610031, China |
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Abstract Objective: Helicobacter pylori candidate vaccine was constructed based on yeast surface display technology and its immunogenicity was further analyzed. Methods: Surface displayed S.cerevisiae EBY100/pYD1-VacA was constructed that vacA gene of Helicobacter pylori as used a research subject. S.cerevisiae EBY100/pYD1-VacA was detected by Western blot analysis,immunofluorescence assay and flow cytometry assay. BALB/c mice of SPF grade were administrated orally with that PBS and S.cerevisiae EBY100/pYD1 were used as the control groups, and S.cerevisiae EBY100/pYD1-VacA was used as the experimental group. VacA-specific sera IgG and secretory IgA titers were determined by ELISA assay. Results: The VacA antigen protein was successfully displayed on the surface of S.cerevisiae EBY100. S.cerevisiae EBY100/pYD1-VacA could produce a higher VacA specific antibodies. Conclusion: Surface displayed yeast can be used as a delivery vector of Helicobacter pylori candidate vaccine. Meanwhile, it will provide new ideas for developing bacteria or virus vaccines.
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Received: 24 December 2019
Published: 02 June 2020
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Corresponding Authors:
Han LEI
E-mail: hlei@swjtu.edu.cn
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