S100A9 Is Involved in <i>Fusobacterium nucleatum</i>-Induced Proliferation and Migration of Colon Cancer HCT116 and SW480 Cells

doi:10.13523/j.cb.1905001

China Biotechnology

2020, Vol. 40

Issue (1-2): 84-91 DOI: 10.13523/j.cb.1905001

Orginal Article

S100A9 Is Involved in Fusobacterium nucleatum-Induced Proliferation and Migration of Colon Cancer HCT116 and SW480 Cells

HU Li-jun¹,DUAN Liang²,HUANG Yi-yun¹,LIN Lu¹,HUANG Mao¹,CHEN Lu¹,PENG Qi¹,HU Qin¹,ZHANG Yan¹,ZHOU Lan^1,^**(

)

1 Key Laboratory of Laboratory Medical Diagnostics of Ministry of Education, Chongqing Medical University, Chongqing 400016, China
2 Department of Laboratory Medicine, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China

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Abstract

Objective: To investigate the effect of S100A9 in proliferation and migration in Fusobacterium nucleatum (Fn)- induced colon cancer HCT116 and SW480 cells. Method:CCK8 assay and Transwell assays were used to detect the proliferation and migration of colon cancer HCT116 and SW480 cells infected with Fn. Real time PCR and Western blot were used to detect expression of S100A9 mRNA and protein in HCT116 and SW480 cells infected with Fn, respectively.After transfecting S100A9 siRNA into cells for silencing S100A9 expression, CCK8 assay and Transwell assays were used to detect the changes of proliferation and migration of HCT116 and SW480 cells infected with Fn. After pre-treatment with NF-κB inhibitor BAY 11-7082, Real time PCR and Western blot were used to detect the changes of S100A9 mRNA and protein in HCT116 and SW480 cells infected with Fn. Result:(1)Fn promotes the proliferation and migration of HCT116 and SW480 cells(P<0.001).(2) The levels of S100A9 mRNA and protein in Fn-infected HCT116 and SW480 cells were significantly higher than that in the control group(P<0.01 or P<0.001), suggesting that S100A9 expression is up-regulated by Fn. (3)The proliferation and migration of Fn- infected HCT116 and SW480 cells were e rescued after silencing S100A9 expression(P<0.001),indicating that S100A9 is involved in it. (4)NF-κB inhibitor effectively inhibited the increase of S100A9 expression induced by Fn. Conclusion: Fn can regulate S100A9 expression by affecting NF-κB activation, and S100A9 is involved in Fn-mediated proliferation and migration of colon cancer cells.

Key words： Colon cancer S100A9 Fusobacterium nucleatum NF-κB;

Received: 03 May 2019 Published: 27 March 2020

ZTFLH:

Q819

Corresponding Authors: Lan ZHOU E-mail: zhoulan@cqmu.edu.cn

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	Li-jun HU
	Liang DUAN
	Yi-yun HUANG
	Lu LIN
	Mao HUANG
	Lu CHEN
	Qi PENG
	Qin HU
	Yan ZHANG
	Lan ZHOU

Cite this article:

HU Li-jun,DUAN Liang,HUANG Yi-yun,LIN Lu,HUANG Mao,CHEN Lu,PENG Qi,HU Qin,ZHANG Yan,ZHOU Lan. S100A9 Is Involved in Fusobacterium nucleatum-Induced Proliferation and Migration of Colon Cancer HCT116 and SW480 Cells. China Biotechnology, 2020, 40(1-2): 84-91.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.1905001 OR https://manu60.magtech.com.cn/biotech/Y2020/V40/I1-2/84

Fig.1 Effect of Fn on proliferation (a) and migration (b) of HCT116 and SW480 cells (a)CCK8 (b)Transwell ** P<0.01; *** P<0.001 (n=3)

Fig.2 Effect of Fn on S100A9 expression in HCT116 and SW480 cells (a)Real time PCR (b)Western blot ** P<0.01; *** P<0.001(n=3)

Fig.3 Effect of down-regulating of S100A9 expression on proliferation (a) and migration (b) of HCT116 and SW480 cells infected with Fn (a)CCK8 (b)Transwell *** P<0.001

Fig.4 Fn up-regulated S100A9 expression via activating NF-κB signaling pathway (a)Real time PCR (b)Western blot ** P<0.01; *** P<0.001


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