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Recombinant Plasmid pcDNA3-dnaJ Prime/DnaJ Protein Boost Immunization Induce Th1/Th17 Immune Responses and Protect Mice Against Pneumococcal Infection |
SUN Si1,QIU Yu-lan2,YAN Ju-rong1,YANG Jing1,WU Guang-ying1,WANG Lin1,XU Wen-chun1,**() |
1 Key Laboratory of Diagnostics Medicine Designated by the Chinese Ministry of Education,Chongqing Medical University, Chongqing 400016, China 2 Laboratory of Nuclear Medicine, Children’s Hospital of Chongqing Medical University, Chongqing 400016, China; |
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Abstract Objective: To explore more effective S. pneumoniae DNA vaccines and vaccine immunization strategies and explore their protective mechanisms.Methods: The recombinant plasmid pcDNA3-dnaJ was constructed and DnaJ protein was expressed. The recombinant plasmid pcDNA3-dnaJ/protein DnaJ immunized group and the plasmid pcDNA3-dnaJ immunized group were separately set to compare the nasal lavage of mice treated with S. pneumoniae strain. The bacterial load and survival rate of the liquid, serum antibody titer and inflammatory factors after challenged were measured by ELISA. The activation of BMDCs and the immune responses of Th1 and Th17 cells were analyzed by flow cytometry.Methods: The plasmid pcDNA3-dnaJ immunization three times induced antigen-specific antibody in serum and reduced the bacterial loads in the nasopharynx after challenge with live S. pneumoniae, but it was less effective in protecting against a lethal infection. However, compared with repeating the plasmid DNA innoculation three times, the strategy of pcDNA3-dnaJ prime one time/DnaJ protein boost one time could significantly reduce the pneumococcal colonization in the nasopharynx and provid better protection against lethal infection. Furthermore, DnaJ protein boosting generated higher levels of IFN-γ and IL-17A than the DNA boosting.Conclusion: Compared with DNA plasmid booster, immunization using DNA prime/protein boost of pneumococcus protein may be a new strategy to develop vaccines against pneumococcal infection.
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Received: 19 April 2019
Published: 15 January 2020
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Corresponding Authors:
Wen-chun XU
E-mail: xuwen@cqmu.edu.com
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