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shPLCε Inhibits Serine/Glycine Metabolism and Proliferation of Prostate Cancer via YAP Signaling Pathway |
DUAN Li-mei1,YANG Jin-xiao1,LIU Jia-yu2,ZHENG Yong-bo2,WU Xiao-hou2,LUO Chun-li1,**() |
1 Key Laboratory of Clinical Diagnostics Founded by Ministry of Education, College of Laboratory,Chongqing Medical University, Chongqing 400016, China 2 The First Affiliated Hospital of Chongqing, Medical University, Chongqing 400016, China |
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Abstract Objective: To investigate the effects of phospholipase C epsilon on serine/glycine metabolism and cell proliferation in prostate cancer cells.Methods: Lentivirus and plasmid were transfected into LNCAP and PC3 cells. The expression of YAP, serine/glycine producing enzyme (PSAT1,PSPH,SHMT2) and proliferation-related genes (Cyclin D1,PCNA) were detected by q-PCR and Western blot. The cloning formation experiment and MTT assays were used to detect the clone formation rate and cell proliferation activity.Results: (1) Infection with LV-shPLCε significantly down-regulated the mRNA and protein levels of PLCε,YAP,serine/glycine producing enzymes (PSAT1,PSPH,SHMT2) and proliferation genes (Cyclin D1,PCNA) in prostate cancer cells LNCAP and PC3. At the same time, it inhibits the clone formation ability and proliferative activity of LNCAP and PC3 cells. (2) After adding over-expressing YAP plasmid to shPLCε group, YAP,serine/glycine producing enzymes and proliferation genes were significantly reversed. but the results of the interference with the down YAP plasmid were reversed.Conclusion: shPLCε inhibits the serine/glycine metabolism and proliferation in prostate cancer cells by down-regulating the expression of YAP.
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Received: 15 April 2019
Published: 17 December 2019
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Corresponding Authors:
Chun-li LUO
E-mail: luochunli79@126.com
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