| Orginal Article |
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| Diagnostic Value of CD11c Antigen in Patients with Chronic Lymphocytic Leukemia |
ZHAO Si-shu,LIU Lu,LIU Fang,QIU Hai-rong,FAN Lei,LI Jian-yong,WU Yu-jie( ) |
| The First Affiliated Hospital with Nanjing Medical University,Jiangsu Province Hospital,Hematology Department,Nanjing 210029,China |
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Abstract Objective: To explore the expression of CD11c antigen in patients with chronic lymphocytic leukemia(CLL),and its clinical diagnostic value,as well as the correlation of CD11c with genetic abnormalities.Methods: 200 cases of CLL,49 cases of MCL were detected for CD11c expression rate and mean fluorescence intensity(MFI)by Multi parameter flow cytometry (FCM).And the relationship between CD11c expression and prognostic markers (ZAP-70 and CD38) expression in 200 CLL patients were compared.At the same time,the fluorescence in situ hybridization(FISH) was used to detecte P53 deletion, 13q14 deletion, ATM deletion, 6q23 deletion,+12 and IGH rearrangement.Then the genetic abnormalities were compared between CD11c - and CD11c + CLL patients. Results: The CD11c positive rate in CLL patients was 49.5%(99/200),MFI median 2.06(1.00 to7.34).While the CD11c positive rate in MCL patients was 6.12%(3/49).MFI median 2.00(1.97 to 2.54).The positive rate of CD11c expression were significantly higher in CLL patients than in MCL patients(x 2=30.62,P<0.05).The positive rate of ZAP-70 and CD38 were significantly higher in CD11c +CLL patients than CD11c -CLL patients(x 2=15.472,P<0.05;x 2=11.556,P<0.05). The results of P53 deletion, 13q14 deletion,ATM deletion,6q23 deletion,+12 and IGH rearrangement were all not significantly different between CD11c - and CD11c + CLL patients. Conclusion: CD11c has significant value for assistant diagnosis of CLL,especially for the differential diagnosis between CLL and MCL.
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Received: 22 August 2019
Published: 20 September 2019
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Corresponding Authors:
Yu-jie WU
E-mail: wuyujiel23456@163.com
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