Immunogenicity of Recombinant Fowl-pox Vaccines coexpressing Foot-and-Mouth Disease virus 3C、P1-2A gene and porcine interleukin 18

China Biotechnology

2007, Vol. 27

Issue (8): 25-28 DOI:

Immunogenicity of Recombinant Fowl-pox Vaccines coexpressing Foot-and-Mouth Disease virus 3C、P1-2A gene and porcine interleukin 18

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Abstract

Objective：Evaluate the immunogenicity of an recombinant fowl-pox virus rFPV-3C-P1-2A-IL-18. Methods：Mice were inoculated with the recombinant FPV(rFPV) rFPV-3C-P1-2A-IL-18 、rFPV-P1-2A-3C vaccine, 282E4 FPV and PBS was set as the control groups.The FMDV specific antibody、number of T subtype 、cytotoxicity of special CTL were detected. Results：Compared with other control groups, the mice inoculated with rFPV-3C- P1-2A-IL-18、rFPV-P1-2A-3C vaccines were induced higher anti-FMDV antibody titer, alarger number of T subtype, and higher cytotoxicity of special CTL. Compared with rFPV-P1-2A-3C vaccine，rFPV-3C-P1-2A-IL-18 can induce larger number of T subtype and higher cytotoxicity of special CTL. Conclusion：It is evident that the recombinant fowl-pox virus rFPV-3C- P1-2A-IL-18 show excellent immunogenicity in mice , thus provide valuable support for further development of FMDV genetic engineering vaccines.

Key words： FMDV recombinant FPV(rFPV) immunogenicity

Received: 27 March 2007 Published: 25 August 2007

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. Immunogenicity of Recombinant Fowl-pox Vaccines coexpressing Foot-and-Mouth Disease virus 3C、P1-2A gene and porcine interleukin 18. China Biotechnology, 2007, 27(8): 25-28.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2007/V27/I8/25

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