High-throughput Sorting Methods for the Selection of Highly Productive Cell Lines Expressing Exogenous Gene

China Biotechnology

2006, Vol. 26

Issue (0): 186-190 DOI:

High-throughput Sorting Methods for the Selection of Highly Productive Cell Lines Expressing Exogenous Gene

Download:

HTML

PDF(432KB) HTML
Export: BibTeX | EndNote (RIS)

Abstract

Screening of the cell population after transfection is the key step to obtain genetically-engineering cell lines with the capability for efficient expression of exogenous gene. The development and application of high-throughput sorting methods has significantly improved the efficiency of cell sorting and expanded the range of screening. It resultantly increased the probability of getting the higher produce cell line. In this article, the mechanism and application of high-throughput sorting methods based on fluorescence-activated cell sorting were reviewed, and the prospect of a novel method based on laser-enabled analysis and processing technology was discussed.

Key words： Mammalian cell Exogenous gene High expression High-throughput sorting Flow cytometric technology

Received: 14 December 2005 Published: 15 June 2006

	Service
	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors

Cite this article:

. High-throughput Sorting Methods for the Selection of Highly Productive Cell Lines Expressing Exogenous Gene. China Biotechnology, 2006, 26(0): 186-190.

URL:

https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2006/V26/I0/186

[1]	WANG Hui-lin,ZHOU Kai-qiang,ZHU Hong-yu,WANG Li-jing,YANG Zhong-fan,XU Ming-bo,CAO Rong-yue. Research Progress of Human Coagulation Factor VII and the Recombinant Expression Systems[J]. China Biotechnology, 2021, 41(2/3): 129-137.

[2]	Yu-lei GUO,Liang TANG,Rui-qiang SUN,You LI,Yi-jun CHEN. High-Throughput Micro Bioreactor Development for Biopharmaceuticals[J]. China Biotechnology, 2018, 38(8): 69-75.

[3]	Xin GAO,Pan-jian WEI,Zhuo-hong YAN,Ling YI,Xiao-jue WANG,Bin YANG,Hong-tao ZHANG. Cloning and Expression of Single Chain Antibody Against Human EGFR[J]. China Biotechnology, 2018, 38(5): 73-78.

[4]	XU Hong-ji, ZHANG Bing-bing. Gene Strategies of Enhancing the Anti-apoptosis Ability of Mammalian Cell in Engineering[J]. China Biotechnology, 2012, 32(6): 104-108.

[5]	HUI Kai-yuan, GAO Xiang-dong, XU Chen. Recent Advances in Cell Engineering for Monoclonal Antibody Production[J]. China Biotechnology, 2012, 32(02): 90-95.

[6]	DIAO Yong. *Inducible Gene Expression of Mammalian via* RNA-only Strategies**[J]. China Biotechnology, 2011, 31(12): 120-125.

[7]	JIAN XU . Retroviral expression system and its application in efficient foreign gene expression[J]. China Biotechnology, 2008, 28(5): 116-121.

[8]	. Advance in Fed-Batch Mammalian Cell Culture[J]. China Biotechnology, 2008, 28(11): 104-109.

[9]	. Cloning, High Expression of Single-Stranded DNA-Binding Protein and Its Interaction with ssDNA[J]. China Biotechnology, 2007, 27(4): 12-17.

[10]	. High Level Prokaryotic Expression of Thymosin α1 by Gene Repeats[J]. China Biotechnology, 2007, 27(1): 11-15.

[11]	. Recombinant baculoviruses as mammalian cell gene-delivery vectors: a review[J]. China Biotechnology, 2007, 27(1): 126-130.

Viewed

Full text

Abstract

Cited

Shared

Discussed