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Prokaryotic expression of OsICK1 gene and anti-OsICK1 antibody preparation |
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Abstract Rice cyclin-dependent kinase inhibitor(ICK)is a key control protein in cell-cycle, it can effect the growth and development of cell. Rice OsICK1 gene was expressed in BL21,with the prokaryotic expression vector pET-28a(+).And anti- OsICK1 polyclonal antibody was obtained by injecting OsICK1 into mouse. The result of Western blotting show that the polyclonal antibody not only can differentially combine with the expressed protein OsICK1, but also can differentially combine with the OsICK1 in rice.It have established the base for the research of OsICK1 gene expression in rice.
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Received: 27 February 2006
Published: 15 June 2006
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