Abstract An improved SDS method was used to extract genomic DNA from Streptomyces gilvosporeus. The RAPD conditions were optimized by single factor experiment and orthogonal experiment, including Taq DNA polymerase, Mg2+, primer , dNTPs and template DNA. The results were as follows: DNA 60-150 ng, Taq DNA polymerase 1.0-1.5U, primer concentration 0.3-0.4 mmol/L, dNTPs concentration 200-250 μmol/L, Mg2+ concentration 2.5-3.0 mmol/L in 20μL PCR system. Under above optimal conditions,the abundant, stable and clear strips could be obtained.
|
