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| Screening and Application of Acid-resistant Strains of Propionibacterium acidipropionici |
| SUN Dan1, XU Guo-xia2, WANG Zi-qiang2, WANG Yun-shan2, ZHANG Li-ping1, SU Zhi-guo2 |
1. College of Life Science, Hebei University, Baoding 071002, China;
2. The State Key Lab of Biochemical Engineering, The Institute of Process Engineering of the Chinese Academy of Sciences, Beijing 100190, China |
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Abstract In order to relieve the inhibitory effect of the metabolites (propionic acid) on the growth of the cells during the production of propionic acid by microbial fermentation, the laboratory storage of Propionibacterium acidipropionici (resistant to 30g/L propionic acid) P-0 as the original strain, a strain of P-10 with good acid-producing ability of 10g/L propionic acid was obtained by propionic acid pressure screening, which reduced the inhibitory effect of propionic acid on cell growth during fermentation.The strain P-10 was subjected to shake flask fermentation, the fermentation cycle was 168h, the concentration of propionic acid was 49.66g/L, the rate of acid production was 0.30g/(L·h), which was 53.04% higher than that of the strain P-0. The experiment of 7L fermentor showed that the fermentation period of the strain P-10 was 168h, the concentration of propionic acid was 55.63g/L, the acid production rate was 0.33g/(L·h). At the same time, the second inoculation experiment was carried out on the strain P-10. The results showed that the optimum period was 84h, and the concentration of propionic acid increased by 17.77% at the second time. The secondary inoculation experiment was not only beneficial to the organic acid but also can improve the acid production capacity and acid resistance of the strains. The tested strain P-10 has excellent acidic stability, which is beneficial to the industrial production and application of the strain and is of great significance to the subsequent fermentation separation and coupling.
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Received: 24 April 2017
Published: 15 November 2017
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