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| Transcriptomic Analysis of Response to Phenol of Evolved and Unevolved Chlorella Strains |
| ZHOU Lin1,2, WANG Liang2, GAO Juan1, ZHAO Quan-yu2, WEI Wei2, SUN Yu-han2 |
1. School of Life Science, Shanghai University, Shanghai 200444, China;
2. Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, China |
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Abstract Phenol is a typical environmental pollutant. Chlorella sp. is potential strain for wastewater treatment because of its fast growth and strong resistance. Chlorella sp. could degrade phenol in industrial wastewater but oxidative stress induced by high concentration of phenol could cause oxidative damage in algal cells. Adaptive evolution was performed to improve the tolerance to phenol of Chlorella sp. (L5) in previous study. The response mechanism of Chlorella sp. to oxidative stress induced by high concentration phenol was explored by de novo comparative transcriptomic analysis on genome scale. It was shown that the evolved strain could tolerate and degrade phenol was related to metabolic regulations in multiple pathways. The results of de novo comparative transcriptomic analysis showed that the genes related to signal transduction, ABC transporter and heat shock protein were significantly up-regulated at 500mg/L phenol concentration compared to those in the original (L3) cells. Those genes in nitrogen metabolism and tricarboxylic acid cycle (TCA) were also upregulated. The evolved strain (L5) could reduce oxidation pressure induced by high concentration of phenol through the metabolic regulations in these metabolic pathways.
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Received: 20 January 2017
Published: 25 July 2017
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