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| Metabolic Engineering of Klebsiella pneumoniae for the Production of Poly(3-Hydroxypropionate) from Glycerol |
| DOU Yi-han1, LI Ying2, ZHAO Peng1, FAN Ru-ting1, TIAN Ping-fang1 |
1. College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China;
2. College of Biochemical Engineering, Beijing Union University, Beijing 100023, China |
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Abstract Poly(3-hydroxypropionate) (P3HP) represents a novel biodegradable and biocompatible polyhydroxyalkanoate. None of currently identified organisms can naturally synthesize P3HP. Two recombinant Klebsiella pneumoniae strains for the production of P3HP were constructed. The propionaldehyde dehydrogenase gene (pduP) from Salmonella enterica serovar typhimurium LT2 and the polyhydroxyalkanoate synthase gene (phaC) from Ralstonia eutropha H16 were cloned by PCR and cloned into vectors. Transformation of vectors into competent K. pneumoniae cells led to two recombinant strains:K. p(pET-tac-pduP-phaC), whereby pduP and phaC shared tac promoter, and K. p(pET-tac-pduP-tac-phaC), whereby pduP and phaC were independently expressed under tac promoter. Using glycerol as the sole carbon source for shake flask fermentation, the strain K. p(pET-tac-pduP-phaC) produced 0.054 g/L of P3HP, by contrast, the strain K. p(pET-tac-pduP-tac-phaC) produced 0.091 g/L of P3HP.
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Received: 27 December 2016
Published: 25 June 2017
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