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中国生物工程杂志

China Biotechnology
China Biotechnology  2016, Vol. 36 Issue (11): 90-97    DOI: 10.13523/j.cb.20161113
    
Engineering Escherichia coli to Synthesize Free Fatty Acids: A Recent Progress
FANG Li xia, CAO Ying xiu, SONG Hao
School of Chemical Engineering and Technology, Key Laboratory of Systems Bioengineering(Ministry of Education), SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering(Tianjin), Tianjin University, Tianjin 300072, China
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Abstract  

Fatty acids are key platform compounds, which are easily transformed to alka(e)ne, ester, fatty alcohols and other widely applied products in energy and chemical industries. Microbial biosynthesis of free fatty acids (FFAs) offers a sustainable and green supplement to fossil or plant/animal based products, attracting extensive research in the last decade. The abundant information of fatty acid metabolism in Escherichia coli enabled diverse metabolic engineering strategies to improve FFAs accumulation from trace amount to about 9g/L, demonstrating E. coli as a promising host for the microbial production of fatty acids. Novel synthetic biology strategies, such as the dynamic feedback regulation by malonyl-CoA sensor-actuator and in vitro reconstitution of the fatty acid synthase, have been exploited for FFAs production in engineered E. coli. In addition, brand-new or more efficient FFAs biosynthesis routes or products were also developed by reversing the β-oxidation cycle and reconstructing multiplex pathways from various species. Recent progresses of FFAs biosynthesis in recombinant E. coli through metabolic engineering and synthetic biology were summarized, and its future trend was explored.



Key wordsMetabolic engineering      Engineered microorganisms      Synthetic biology      Fatty acids      Escherichia coli     
Received: 31 March 2016      Published: 25 November 2016
ZTFLH:  Q819  
Cite this article:

FANG Li xia, CAO Ying xiu, SONG Hao. Engineering Escherichia coli to Synthesize Free Fatty Acids: A Recent Progress. China Biotechnology, 2016, 36(11): 90-97.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20161113     OR     https://manu60.magtech.com.cn/biotech/Y2016/V36/I11/90

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