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中国生物工程杂志

China Biotechnology
China Biotechnology  2016, Vol. 36 Issue (9): 1-10    DOI: 10.13523/j.cb.20160901
    
The character of cervical cancer patients and healthy women in experiment group and validation group
AN Long-fei1, JIN Long2, SUN Feng-liang4, LI Kai3, YAN Cheng-zhi4, QIN Jun3, ZHANG Pu-min3, WU Chen1, CHEN Huan3
1 College of Life Sciences, Hebei University, Baoding 071002, China;
2 Department of Oncology Radiotherapy, Shanxi Province People's Hospital, Xi'an 710068, China;
3 Beijing Proteome Research Center, Beijing 102200, China;
4 Department of Urology Surgery, Baodi Clinical Hospital, Tianjin Medical University, Tianjin 301800, China
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Abstract  

To explore the differential proteome pattern and screen biomarkers in urine samples from cervical cancer patients, urine samples from 43 patients suffering from cervical cancer (CC) and 47 healthy women (HW) were collected for further proteomic analysis. Urine proteins precipitated by ultracentrifugation were subjected to SDS-PAGE separation and LC-MS/MS (liquid chromatograph-mass spectrometer/mass spectrometer) analysis by using the label-free protein quantification assay. By comparing the urine proteomes between CC, HW and cervical cancer tissue groups,over 1910 proteins were mutually exclusive in CC and HW groups, while 746 proteins were found in all three groups. In the subset of 746 proteins, 84 up-regulated proteins and 82 down-regulated proteins in CC group when compared with the HW group. Further bioinformatics analysis showed that S100A7(S100 calcium binding protein A7) and CEACAM8(carcinoembryonic antigen-related cell adhesion molecule 8) were specific to CC. For further validation, LC-MS/MS was applied quantification on 70 samples. The combined analysis of S100A7 and CEACAM8 (S100A7+CEACAM8) can improve the sensitivity of cervical cancer diagnosis from 73% (S100A7) and 87% (CEACAM8) to 93%. The urine proteome of cervical cancer was different from urine proteome of healthy women be envied. S100A7 and CEACAM8 were potential biomarkers for cervical cancer.



Key wordsUrine proteome      Crvical cancer      Biomarker     
Received: 15 February 2016      Published: 25 September 2016
ZTFLH:  Q816  
Cite this article:

AN Long-fei, JIN Long, SUN Feng-liang, LI Kai, YAN Cheng-zhi, QIN Jun, ZHANG Pu-min, WU Chen, CHEN Huan. The character of cervical cancer patients and healthy women in experiment group and validation group. China Biotechnology, 2016, 36(9): 1-10.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20160901     OR     https://manu60.magtech.com.cn/biotech/Y2016/V36/I9/1

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