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| Scale-up Process Optimization for Recombinant PPV-VP2 Protein Production Using Baculovirus Expression System in 40L Bioreactor |
| SU Xiao-rui, LI Wei-guo, WANG Yan-hui, GAO Xiao-jing, SHAN Yi-hong, TAN Fei-fei, LI Xiang-dong, TIAN Ke-gong |
| National Research Center for Veterinary Medicine, Luoyang 471003, China |
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Abstract The focuses are on the scale-up of a Sf9 cell line to produce recombinant porcine parvovirus (PPV) VP2 protein expression using baculovirus/insect expression system from the initial 3-L bench scale to the 40-L scale. In 3L bioreactor, HA titer of VP2 protein side-by-side comparison of shake flask by optimization of DO and Agit. The operational parameters of temperature, DO, and pH for large vessels were set at the same values as those of 3-L bioreactor. Appropriately applying the calculated results to power input per volume, oxygen transfer coefficient and tip speed, resulted in successful scale-up of agitation speed for the large bioreactors. By HA titer analysis, VP2 protein had identical haemagglutinating activity comparison of 3-L. By guinea pig immunization test, It is found that recombinant VP2 subunit vaccine can be induced high level antibody reaction, and immune with recombinant VP2 subunit vaccine was faster than classical inactivated PPV vaccine.
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Received: 19 March 2017
Published: 25 October 2017
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