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| Research Progress on Cloning, Expression,Immobilization and Molecular Modification of Nitrilase |
| LI Ji-bin, CHEN Zhi, CHEN Hua-you |
| Institute of Life Science, Jiangsu University, Zhenjiang 212013,China |
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Abstract With the rapid development in genetic engineering technology, the analysis of the nitrilase gene from different strains, was cloned in the expression strain, to construct an efficient and stable genetically engineered bacteria. The molecular modification of the nitrilase could significantly improve the activity stability, substrate tolerance and substrate specificity of the enzyme, which make possible the use of nitrile hydrolase in the industrial applications.The origin, structure, catalytic mechanism, clonal expression, immobilization, molecular modification and prospects of nitrilase were reviewed. And it demonstrate important guidelines for the significance of nitrilase research.
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Received: 08 March 2017
Published: 25 September 2017
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