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中国生物工程杂志

China Biotechnology
China Biotechnology
China Biotechnology  2016, Vol. 36 Issue (7): 112-116    DOI: 10.13523/j.cb.20160715
    
Establishment and Application of Genetic Motification System for Mortierella alpina
ZENG Si-yu1, SHI Tian-qiong1, SHI Kun1, REN Lu-jing1, HUANG He1,2, JI Xiao-jun1
1 State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing Tech University, Nanjing 211816, China;
2 College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 210009, China
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Abstract  

Mortierella alpina is a significant oil-producing fungi in which oil content reached up to 50% of dry cell weight. It contains a wide range of polyunsaturated fatty acids and its content is higher than other strains. Recently, molecular biology research for Mortierella alpina genetic modification system has made great progress. The establishment of Mortierella alpina genetic modification system is important to improve the level of polyunsaturated fatty acids. The latest application of genetic modification system for Mortierella alpina was reviewed, including transformation methods, types of selective markers, their advantages and disadvantages, and the application of the transformation system.

Key words： Mortierella alpina      Selective markers      Polyunsaturated fatty acids      Transformation methods      Genetic modification system     
Received: 27 March 2016      Published: 25 July 2016
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Cite this article:

ZENG Si-yu, SHI Tian-qiong, SHI Kun, REN Lu-jing, HUANG He, JI Xiao-jun. Establishment and Application of Genetic Motification System for Mortierella alpina. China Biotechnology, 2016, 36(7): 112-116.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20160715     OR     https://manu60.magtech.com.cn/biotech/Y2016/V36/I7/112

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