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中国生物工程杂志

China Biotechnology
China Biotechnology  2016, Vol. 36 Issue (7): 55-63    DOI: 10.13523/j.cb.20160709
    
Screening of the Protein Related to Antibiotics Biosynthesis in Micromonospora carbonacea JXNU-1
CHEN Juan, YANG Hui-lin, HUANG Yun-hong, LONG Zhong-er
College of Life Sciences, Jiangxi Normal University, Nanchang 330022, China
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Abstract  

Objective:To screen the protein related to antibiotics biosynthesis in Micromonospora carbonacea JXNU-1 with broad-spectrum antimicrobial activity. Methods:iTRAQ was used to analyze the differentially expressed proteins between the time of antibiotics secretion/non-secretion in M.carbonacea JXNU-1. Results:A total of 2 390 proteins were identified using iTRAQ, and 172 proteins were differentially expressed during the antibiotics secretion time in M.carbonacea JXNU-1, including 76 up-regulated and 96 down-regulated. Twelve proteins and five gene clusters closely related to biosynthesis of antibiotics were screened based on the functional analysis of the differentially expressed proteins with GO and COG, annotation. Conclusion:The proteins related to antibiotics biosynthesis in M.carbonacea JXNU-1 have been screened based on iTRAQ, it could provide the theoretical basis for revealing the mechanism of antibiotics biosynthesis in M.carbonacea JXNU-1.



Key wordsAntibiotic      Micromonospora carbonacea      Biosynthetic pathway      Gene cluster      Proteomics     
Received: 27 January 2016      Published: 25 July 2016
ZTFLH:  Q591.2  
Cite this article:

CHEN Juan, YANG Hui-lin, HUANG Yun-hong, LONG Zhong-er. Screening of the Protein Related to Antibiotics Biosynthesis in Micromonospora carbonacea JXNU-1. China Biotechnology, 2016, 36(7): 55-63.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20160709     OR     https://manu60.magtech.com.cn/biotech/Y2016/V36/I7/55

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