中国生物工程杂志

The thalassemia is a heterogeneous group of inherited disorders. The main pathological mechanism which causes thalassemia is the imbalance in the synthesis of αandβglobin chains, due to mutations in the globin loci. The C→T transition at nucleotide 654 of intron 2 inβglobin gene is one of the most frequent βthalassemia mutation(β-IVSⅡ-654) in GuangDong China. The expression of human β- globin gene cluster is definitely regulated with strict developmental stage and tissue lineage specificities as well as the delicated control of the biosynthetic equilibrium. It was all know that the expression of human β- globin gene cluster concerned with the β locals control region(βLCR) in human being. Purpose：We wanted to identify that what contribution wouldβLCR give toβ thalassemia gene (β-IVSⅡ-654) in the transgenic mouse. Methods: To prepare two genes, one was the merelyβthalassemia gene, the other was a modified βthalassemia gene contains βLCR. To made up two lines of transgenic mouse by use these two genes. Then, detect the expression of two genes in the two transgenic mouse lines by fluorescent quantitation RT-PCR. To compared the two expressions of two genes by statistical analysis. Result: Two transgenic mouse were established. The statistical analysis shows that the expression of the merelyβthalassemia gene was more lower than the modified βthalassemia gene contains βLCR in the transgenic mouse. Conclusion: We therefore conclude that a combination ofβLCR is necessary to highly expressionsβthalassemia gene in the transgenic mouse.