中国生物工程杂志

We used differential adherence selection to enrich spermatogonial stem cells (SSCs)from pup mouse testis, and transfer them to recipients pre-treated with busulfan through rete testis. Two months after transplantation, recipient mouse testes were recovered and observed that the transplanted donor cells had colonized into recipient seminiferous tubules. At 113 day after transplantation when recipients were mated with wild-type females of same genetic background, offspring with the donor cells genetic information were produced. This study indicated that the testis cells selected by differential adherence selection not only contained enriched SSCs, but also maintained their stem cell activity，which allows them to colonize recipient testis and restore normal spermatogenesis.