Optimization on Fermentation and Purification of the Fibrinolytic Enzyme from Stenotrophomonas maltophilia

China Biotechnology

2007, Vol. 27

Issue (9): 47-52 DOI:

Optimization on Fermentation and Purification of the Fibrinolytic Enzyme from Stenotrophomonas maltophilia

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Abstract

The optimization on liquid fermentation and purification of the fibrinolytic enzyme from Stenotrophomonas maltophilia（DR-929） was investigated in this paper.The results showed the best fermentation are amidulin 2.0%, soya flour 1.0%, yeast extract 0.5%, NaCl 1.0%, CaCl2 0.02%, MgSO4 0.05%, inoculum of 36 hours, fermental time 4d, initial pH 8.0 or 9.0, temperature 25℃, volume of media 30mL, volume of inoculum 5% or 6%.The purification process includes the following steps: removing cells by the centrifugation, 25%~70% saturation ammonium sulfate precipitation, HIC with Phenyl FF（high sub）, IEC with Q-Sepharose FF, gel filtration chromatography with Superdex 75. SDS-PAGE electrophoresis was used to examine the purification effect, and the results indicated that homogeneous strap in SDS-PAGE and has a molecular weight around 28.3KD.

Key words： Stenotrophomonas maltophilia fibrinolytic enzyme fermentation condition purification

Received: 04 June 2007 Published: 25 September 2007

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. Optimization on Fermentation and Purification of the Fibrinolytic Enzyme from Stenotrophomonas maltophilia. China Biotechnology, 2007, 27(9): 47-52.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2007/V27/I9/47

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