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| Formation of Equol from Dihydrodaidzein by Recombinant Saccharomyces cerevisiae |
| LIU Yu-xue1,2, ZHANG Yi-xin1, WANG Lei1, LIN Xin-ping1, ZHU Zhi-wei1, ZHAO Zong-bao1 |
1. Division of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China;
2. University of Chinese Academy of Science, Beijing 100049, China |
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Abstract Equol is an active and stable phytoestrogen. It can be produced by microbial conversion of a known compound daidzein. A recombinant Saccharomyces cerevisiae strain was constructed, which harbored a plasmid enabling expression of genes orf-1, orf-2 and orf-3 for the conversion of daidzein into equol. It was found that the recombinant strain was able to convert dihydrodaidzein, the immediate downstream product of the reaction sequence, into equol. The results provided valuable information for the construction of yeast cell factory for de novo biosynthesis of equol.
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Received: 11 March 2014
Published: 25 April 2014
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