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Effects of bmy Gene knockout on Hemolysis and Antifungal activity of Bacillus amyloliquefaciens Q-426 |
TAO Si-mei1,2, ZHENG Wei1,2, ZHAO Peng-chao3, ZHOU Wei1,2, QUAN Chun-shan1,2, FAN Sheng-di1,2 |
1. Department of Life Science, Dalian Nationalities University, Dalian 116600, China; 2. SEACME Key laboratory, Dalian 116600, China; 3. Department of Medical Technology and Engineering, Henan University of Science and Technology, Luoyang 471003, China |
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Abstract Iturin family of lipopeptides has strong antifungal activity against pathogenic fungi such as Trichophyton rubrum and has potential to become a new antifungal drug currently used against dermatophytes. Bacillus amyloliquefaciens Q-426 could produce fengycins and bacillomycin D (a member of iturin family), and its fermentation broth shows a hemolytic activity. In order to determine whether bacillomycin D is the main hemolytic substances, a homologous recombinantion gene knockout technology was used to construct strain Q-426-Δ-bmy. Bacillomycin D was not detected in the Q-426-Δ-bmy strain fermentation broth, and there was a significant reduction in the hemolysis and antifungal activity in contrast with wild strain Q-426, indicating that bacillomycin D is closely related to hemolytic activity and antifungal activity of Bacillus amyloliquefaciens Q-426.
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Received: 17 December 2013
Published: 25 March 2014
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