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| The Role of Acid Stress on Improved Performance of Selenium/glutathione-Enriched Candida utilis |
| WANG Da-hui, XU Hong-qing, WANG Cheng-fu, WEI Gong-yuan |
| School of Biology and Basic Medical Sciences, Soochow University, Suzhou 215123, China |
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Abstract Intracellular organic selenium and glutathione contents are the two most important characteristics of high performance of selenium-enriched yeast. In order to improve the performance of selenium/glutathione-enriched Candida utilis, the effects of diverse pH conditions on cell growth, glutathione biosynthesis and the biotransformation of inorganic selenium to organic selenium during selenium enrichment were investigated. It was indicated that the moderate acid stress was favor of the increased intracellular contents of organic selenium and glutathione, as well as the decreased level of the oxidized compound as malondialdehyde. The maximum levels of the intracellular organic selenium and glutathione were obtained at pH 3.5 as 1.13 mg/g and 12.3 mg/g, respectively. Based on the analysis of residual methionine in the medium, intracellular pH, the ratios of NADH/NAD+ and ATP/ADP, the reason of the improved performance of selenium/glutathione-enriched Candida utilis by moderate acid stress were partly interpreted. The results presented will give a feasible approach for further enhanced nutrimental function of Candida utilis.
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Received: 03 September 2013
Published: 25 November 2013
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