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Study on Adsorption Properties of Lycopene from the Broth of Blakeslea trispora by Macroporous Resin |
LI Gan-lu,WU Qi-ci,WU Zhen-jun,SHI Nan,WAN Hong-gui |
Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009,China |
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Abstract The potential of macroporous resin for separation and purification of lycopene produced by B.trispora were investigated. The adsorptiondesorption properties for lycopene of the resins such as HP20, HP2MG, SP825 and SP207 were studied. The optimal resin for lycopene was screened and selected. The results expressed that HP20 resin had a better adsorption-desorption property for lycopene. It could quickly reach an adsorption balance in one hour. Purification of lycopene using packed bed column by HP20 resin was carried out using step gradient of 55% isopropyl alcohol in acetone followed by 65% isopropyl alcohol in acetone. A 89.55% recovery of lycopene was detected by HPLC. After further recrystallisation the purity of lycopene was above 95% and the structure was confirmed by IR, MS and 1H-NMR. The whole process was simple, easy to operate, with a good industrial application value.
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Received: 16 December 2009
Published: 29 April 2010
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