The Process of Methods on Validating the Differential Transcription of Genes

doi:10.13523/j.cb.20150514

China Biotechnology

2015, Vol. 35

Issue (5): 96-102 DOI: 10.13523/j.cb.20150514

The Process of Methods on Validating the Differential Transcription of Genes

ZUO Hai-yang^1,2, CHEN Xiao-li², CAI Yong², HAO Hai-sheng², QIN Tong², ZHAO Xue-ming², LU Yong-qiang³, WANG Dong²

1. Animal Science and Technology College, Jilin Agricultural University, Changchun 130118, China;
2. The Key Laboratory for Farm Animal Genetic and Utilization of Ministry of Agriculture of China, Institute of Animal Science, Chinese Academy of Agricultural Science, Beijing 100193, China;
3. Animal Husbandry Station of Beijing, Beijing 100107, China

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Abstract

The differential expression of genes is closely related to biological characters and function of tissue and cell. With the extensive study on the differentiation of tissue and organs and the growth and development of cells and individuals, the validation of differentially expressed genes are getting more and more important. Thus, a series of validating techniques of differentially expressed analysis were established on the basis of nucleic acid hybridization and PCR, such as Northern blot and fluorescence quantitative PCR. The differentially expressed genes were validated of different treatment, different organs and different development period using these methods, which laid a solid foundation for the gene functional analysis. With the fast development of the detecting methods, the validation methods of differential expression of genes has developed from qualitative to quantitative, from tedious and complicated to simple and rapid, from requiring a large number of initial RNA to only a small amount of RNA, even the fluoresce quantitative PCR method for single cell has been established, and the method is to be more efficient, accurate and low cost. However, The validation for the samples difficult sampling like germ cell and less amount of RNA containing like sperm is still a great challenge for the current method. The validation methods of differential expression of genes from the perspective of the development process was reviewed. Reference to the detedtion of samples dificult sampling and less amount RNA containing was provided.

Key words： Differential expression of gene Fluorescence quantitative PCR Nucleic acid hybridization Nucleic acid probe

Received: 02 February 2015 Published: 25 May 2015

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ZUO Hai-yang, CHEN Xiao-li, CAI Yong, HAO Hai-sheng, QIN Tong, ZHAO Xue-ming, LU Yong-qiang, WANG Dong. The Process of Methods on Validating the Differential Transcription of Genes. China Biotechnology, 2015, 35(5): 96-102.

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https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20150514 OR https://manu60.magtech.com.cn/biotech/Y2015/V35/I5/96

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