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中国生物工程杂志

China Biotechnology
China Biotechnology  2015, Vol. 35 Issue (10): 100-107    DOI: 10.13523/j.cb.20151015
    
Applications and Biodiversity of Prokaryotic Microorganisms with Nitrile Converting Enzymes
WANG Shi-wei, WANG Qing-hui, ZHAI Li-ping, LIU Jun, ZHENG Miao-miao, WANG Fang, YU Zhi-dan
School of Life Science and Agriculture and Forestry, Qiqihar University, Qiqihar 161006, China
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Abstract  

The nitrile converting system can overcome the shortcomings of serious pollutions and unsafe factors of traditional ones, providing new routes for the green chemical engineering and environmental friendly processes. The microbial diversity and biotransformation characteristics of nitrile converting enzymes are presented. NHase gene expression and its tolerance are also discussed.In the near future, an increasing number of novel nitrile converting enzymes will be screened and their potential in the production of useful fine chemicals will be further exploited.



Key wordsGreen chemistry      Nitrile converting enzymes      Advances      Microbial diversity     
Received: 04 May 2015      Published: 25 October 2015
ZTFLH:  Q819  
Cite this article:

WANG Shi-wei, WANG Qing-hui, ZHAI Li-ping, LIU Jun, ZHENG Miao-miao, WANG Fang, YU Zhi-dan. Applications and Biodiversity of Prokaryotic Microorganisms with Nitrile Converting Enzymes. China Biotechnology, 2015, 35(10): 100-107.

URL:

https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20151015     OR     https://manu60.magtech.com.cn/biotech/Y2015/V35/I10/100

[1] Kelly J L, Kornberg H L.Purification and properties of acyltransferases from Pseudomons aeruginosa. Biochemical Journal, 1964, 93:557-568.
[2] 徐建妙,郑裕国,沈寅初.腈水解酶的来源、结构、作用机制及其应用.微生物学通报,2005,32(5):141-146. Xu J M, Zheng Y G, Sheng Y C. The nitrilase: sources, mechanism and applications. Microbiology China, 2005,32(5):141-146.
[3] Asano Y, Fujishiro K, Tani Y, et al. Aliphatic nitrile hydratase from Arthrobacter sp.J-1: purification and characterization. Agricultural and Biological Chemistry, 1982,46:1165-1174.
[4] Prasad S, Bhalla T. Nitrile hydratases (NHases): At the interface of academia and industry. Biotechnology Advances, 2010,28(6):725-741.
[5] Prasad S, Misra A, Jangir V P, et al. A propionitrile-induced nitrilase of Rhodococcus sp. NDB 1165 and its application in nicotinic acid synthesis. World Journal of Microbiology and Biotechnology, 2007,23:345-353.
[6] Cantarella L, Gallifuoco A, Malandra A, et al. High-yield continuous production of nicotinic acid via nitrile hydratase-amidase cascade reactions using cascade CSMRs. Enzyme and Microbial Technology, 2011,48(4-5):345-350.
[7] Pratush A, Seth A, Bhalla T C.Cloning, sequencing, and expression of nitrile hydratase gene of mutant 4D strain of Rhodococcus rhodochrous PA 34 in E. coli. Applied Biochemstry Biotechnology, 2012,168(3):465-486.
[8] Zhang J L, Wang M, Sun H, et al. Isolation and characterization of Rhodococcus ruber CGMCC3090 that hydrolyzes aliphatic, aromatic and heterocyclic nitriles. African Journal of Biotechnology, 2009, 8(20):5467-5486.
[9] Martinkova L, Kren V. Biotransformations with nitrilases. Current Opinion in Chemical Biology, 2010, 14 (2) : 130-137.
[10] Precious S, Goulas P, Duran R. Rapid and specific identification of nitrile hydratase (NHase)-encoding gene in soil samples by polymerase chain reaction. FEMS Microbiology Letters, 2001,204(1):155-161.
[11] Vega-Hernández M C, León-Barrios M, Pérez-Galdona R. Indole-3-acetic acid production from indole-3-acetonitrile in Bradyrhizobium. Soil Biology and Biochemistry, 2002, 34(5):665-668.
[12] Zhou L Y, Zhang L J, Sun S L, et al.Degradation of the neonicotinoid insecticide acetamiprid via the N-carbamoylimine derivate (IM-1-2) mediated by the nitrile hydratase of the nitrogen-fixing bacterium Ensifer meliloti CGMCC 7333. Journal of Agricultural and Food Chemistry, 2014, 62(41):9957-9964.
[13] 曹明乐,姜兴林,张海波,等.一株产腈水解酶的泛菌及其催化特征.应用与环境生物学报,2013,19(2):346-350. Cao M L, Jiang X L, Zhang H B, et al. A new nitrilase producing Pantoea sp. and its enzymological properties. Chinese Journal of Applied and Environmental Biology, 2013,19(2):346-350.
[14] Lievano R, Pérez H I, Manjarrez N, et al. Hydrolysis of ibuprofen nitrile and ibuprofen amide and deracemisation of ibuprofen using Nocardia corallina B-276. Molecules, 2012,17(3):3148-3154.
[15] Wang Y J, Zheng Y G, Xue J P, et al.Characterization of nitrile hydratation catalysed by Nocardia sp. 108. World Journal of Microbiology and Biotechnology, 2007, 23(3):355-362.
[16] Gavagan J E, Fafer S K, Fallon R D, et al. Chemoenzymatic production of lactams from aliphatica, ω dinitriles. Journal of Organic Chemistry, 1998, 63 (14) :4792-4801.
[17] Petrillo K L, Wu S, Hann E C, et al. Over-expression in Escherichia coli of a thermally stable and regio-selective nitrile hydratase from Comamonas testosteroni 5-MGAM-4D. Applied Microbiology and Biotechnology, 2005, 67(5):664-670.
[18] van Pelt S, Zhang M, Otten L G, et al.Probing the enantioselectivity of a diverse group of purified cobalt-centred nitrile hydratases. Organic and Biomolecular Chemistry,2011, 9(8):3011-3019.
[19] Fallon R D, Stieglitz B, Turner I. A Pseudomonas putida capable of stereoselective hydrolysis of nitriles. Applied Microbiology and Biotechnology, 1997, 47(2):156-161.
[20] 郑裕国,金晓峰,郑仁朝,等.一类生物催化剂-氰基耐受型腈水合酶.生物加工过程,2010,8(3):73-78. Zheng Y G, Jin X F, Zheng R C, et al. A class of biocatalyst: cyanide-resistant nitrile hydratase. Chinese Journal of Bioprocess Engineering, 2010, 8(3):73-78.
[21] Cui Y, Cui W, Liu Z, et al.Improvement of stability of nitrile hydratase via protein fragment swapping. Biochemical and Biophysical Research Communications, 2014, 450(1):401-408.
[22] Liu Y, Cui W, Fang Y, et al.Strategy for successful expression of the Pseudomonas putida nitrile hydratase activator P14K in Escherichia coli. BMC Biotechnology, 2013,13(1):48.
[23] Pei X, Wang Q, Li C, et al.Addition of Co2+ to culture medium decides the functional expression of a recombinant nitrile hydratase in Escherichia coli. Biotechnology Letters, 2013, 35(9):1419-1424.
[24] Pei X, Wang Q, Meng L, et al. Chaperones-assisted soluble expression and maturation of recombinant Co-type nitrile hydratase in Escherichia coli to avoid the need for a low induction temperature. Journal of Biotechnology, 2015,203:9-16.
[25] Kuhn M L, Martinez S, Gumataotao N, et al.The Fe-type nitrile hydratase from Comamonas testosteroni Ni1 does not require an activator accessory protein for expression in Escherichia coli.Biochemical and Biophysical Research Communications,2012, 424(3):365-370.
[26] Zhang H J, Zhou Q W, Zhou G C, et al.Biotransformation of the neonicotinoid insecticide thiacloprid by the bacterium Variovorax boronicumulans strain J1 and mediation of the major metabolic pathway by nitrile hydratase. Journal of Agricultural and Food Chemistry, 2012, 60(1):153-159.
[27] Cheng Y M, Ma L, Deng C, et al. Effect of PEG-mediated pore forming on Ca-alginate immobilization of nitrilase-producing bacteria Pseudomonas putida XY4.Bioprocess and Biosystems Engineering, 2014, 37(8):1653-1658.
[28] Chen J, Yu H, Liu C,et al. Improving stability of nitrile hydratase by bridging the salt-bridges in specific thermal-sensitive regions. Journal of Biotechnology, 2012, 164(2):354-362.
[29] Lee C Y, Choi S K, Chang H N. Bench-scale production of acrylamide using the resting cells of Brevibacterium sp. CH2 in a fed-batch reactor. Enzyme and Microbial Technology, 1993, 15(11):979-984.
[30] Nawaz M S, Franklin W, Campbell W L, et al. Metabolism of acrylonitrile by Klebsiella pneumonia. Archives of Microbiology, 1991, 156 (3):231-238.
[31] Fang S, An X, Liu H, et al.Enzymatic degradation of aliphatic nitriles by Rhodococcus rhodochrous BX2, a versatile nitrile-degrading bacterium.Bioresource Technology, 2015,185:28-34.
[32] Kang M S, Han S S, Kim M Y, et al.High-level expression in Corynebacterium glutamicum of nitrile hydratase from Rhodococcus rhodochrous for acrylamide production. Applied Microbiology Biotechnology, 2014, 98(10):4379-4387.
[33] Raj J, Prasad S, Sharma N N, et al.Bioconversion of acrylonitrile to acrylamide using polyacrylamide entrapped cells of Rhodococcus rhodochrous PA-34. Folia Microbiologica (Praha),2010,55(5):442-446.
[34] Luo H L, Fan L, Chang Y, et al. Gene cloning, overexpression, and characterization of the nitrilase from Rhodococcus rhodochrous tg1-A6 in E. coli. Applied Biochemistry and Biotechnology, 2010,160(2):393-400.
[35] Rzeznicka K L, Schätzle S, Böttcher D, et al.Cloning and functional expression of a nitrile hydratase (NHase) from Rhodococcus equi TG328-2 in Escherichia coli, its purification and biochemical characterisation.Applied Microbiology Biotechnology, 2010,85(5):1417-1425.
[36] Kakeya H, Sakai N, Sugai T, et al.Microbial hydrolysis as a potent method for the preparation of optically active nitriles, amides and carboxylic acids. Tetrahedron Letters, 1991, 32(10):1343-1346.
[37] Precigou S, Wieser M, Pommares P, et al.Rhodococcus pyridinovorans MW3, a bacterium producing a nitrile hydratase. Biotechnology Letters, 2004, 26(17):1379-1384.
[38] Lin Z J, Zheng R C, Wang Y J, et al.Enzymatic production of 2-amino-2,3-dimethylbutyramide by cyanide-resistant nitrile hydratase. Journal of Industrial Microbiology and Biotechnology, 2012,39(1):133-141.
[39] 钟莉萍, 张锦丽, 宋洋,等. 一株腈水合酶产生菌的筛选、鉴定及培养条件研究. 天津科技大学学报, 2008, 23(4):35-39. Zhong L P. Zhang J L, Song Y. et al. Study on screening, identification and culture conditions of a strain with nitrile hydratase. Journal of Tianjin University of Science and Technology, 2008, 23(4):35-39.
[40] 宋洋, 钟莉萍, 张锦丽,等.金属离子对红球菌腈水合酶活力的影响. 天津科技大学学报, 2009, 24(1): 11-14,19. Song Y, Zhong L P, Zhang J L, et al. Effect of metal ions on Rhrodococcus sp. nitrile hydratase activity. Journal of Tianjin University of Science and Technology, 2009, 24(1): 11-14,19.
[41] 张锦丽, 王敏, 钟莉萍,等. Rhodococcus ruber TCCC28001产丙烯腈水合酶过程调节及转化条件研究.山东农业大学学报: 自然科学版, 2010, 1: 11-16. Zhang J L, Wang M, Zhong L P, et al. Identification and study on enzymic activity of Rhodcoccus sp.TCCC28001 with nitrile hydratase activity. Journal of Shangdong Agricultural University (Natural Science), 2010,41(1):11-16.
[42] Wang S W, Wang M, Wang Q H. Breeding of NHase hyper-producing Rhodococcus ruber strain LUV30-06 and verification of mutants by RAPD. American Journal of Molecular Biology, 2013,3(2):108-114.
[43] Wang S W, Wang Q H, Zhai Y, et al. Screening of NHase high-Producing Rhodococcus ruber Strain TQD-58 by Both Parents Inactivated Protoplast Fusion. In Zhang X W, Liu H, Liu P U. Proceedings of ITME 2012. 2012 International Symposium on Information Technology in Medicine and Education (ITME2012), Hokkaido, Japan.2012, 737-740.
[44] Shen Y B, Wang M, Li X D, et al. Highly efficient synthesis of 5-cyanovaleramide by Rhodococcus ruber CGMCC3090 resting cells. Journal of Chemical Technology and Biotechnology, 2012, 87(10):1396-1400.
[45] 王世伟, 王卿惠, 王惟熠,等. 腈水合酶蛋白质纯化的研究进展.高师理科学刊, 2013, 33(1): 50-53. Wang S W, Wang Q H, Wang W Y, et al. Research process on protein purification of nitrile hydratase. Journal of Science of Teachers' College and University, 2013, 33(1): 50-53.
[46] Wang S W, Dai Y J, Wang J X, et al. Molecular insights into substrate specificity of Rhodococcus ruber CGMCC3090 by gene cloning and homology modeling. Enzyme and Microbial Technology, 2013, 52(2):111-117.
[47] 王世伟,王敏.腈类物降解菌多样性和产腈水合酶研究进展.中国生物工程杂志,2011,31(9):117-123. Wang S W, Wang M. Research process on microbial diversity of producing-nitrile hydratase and study on nitrile hydratase. China Biotechnology, 2011, 31(9);117-123.

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