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| Primer Spanner: A Web-based Platform to Design PCR Primers for High Efficient Site-directed Mutagenesis |
| PEI Zhi-yong1,2, HOU Xian-hui3,4, GUI Xiao-ke1, CHEN Yu-bao1 |
1. Beijing Computing Center, Beijing Academy of Science and Technology, Beijing 100094, China;
2. Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China;
3. Beijing Nuclear Magnetic Resonance Center, Peking University, Beijing 100871, China;
4. College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China |
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Abstract PCR-based strategy provides economical and efficient application in site-directed mutagenesis (SDM) and DNA assembling such as PCR based vector construction. In these processes, primers are extremely important as they determing amplification efficiency of the target DNA fragments,also influencing circularization of the new plasmids. So this web-based software Primer Spanner (PS) was developed to design primer pairs with partially complementary 5'-ends, which is a special tool for chimeric primer designing. Primers designed by this tool are applied to substitution, insertion and deletion for single-site or multi-site mutagenesis. The performance of the software had been verified by designed experiment and DNA sequencing. The PS primer designing engine is freely accessible at: http://PS1.biocloud.org.cn.
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Received: 05 June 2015
Published: 25 October 2015
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