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| Application of Phosphate Solubilizing Yeast Pichia farinose FL7 in Phytoextraction of Nickel Contaminated Soil |
| HU Zong-fu, ZHU Hong-ji |
| Key Laboratory of Systems Bioengineering, Ministry of Education, Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China |
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Abstract A strain of phosphate solubilizing yeast Pichia farinose FL7 that exhibited strong solubilizing capacity to calcium, aluminum and iron phosphate was studied for its application in the phytoextraction of nickel contaminated soil. The phosphate solubilizing capacity to Ni3(PO4)2 and the nickel tolerance to Ni(NO3)2 of P. farinose FL7 were characterized under liquid conditions. Then P. farinose FL7 bioinoculant was prepared and used in the phytoextraction of Ni(NO3)2 and Ni3(PO4)2 contaminated soil with Brassica juncea as the hyperaccumulator. P. farinose FL7 could directly solubilize PO43- from Ni3(PO4)2 more than 10 folds greater than the control without inoculation and showed higher nickel tolerance. The results of greenhouse pot experiment revealed that the addition of P. farinose FL7 significantly increased Ni accumulation in B. juncea. The total biomass of B. juncea was promoted by the inoculation treatment, which increased from (0.23±0.01)g to (0.34±0.01)g. The changes of soil available phosphate and nickel speciation under different treatments indicated that inoculation of P. farinose FL7 increased the immobilization of soluble nickel in Ni(NO3)2 spiked soil and that biofertilization also increased the bioavailability of insoluble nickel in Ni3(PO4)2 spiked soil. P. farinose FL7 showed dual function and could resulted in improved decontamination rates during the microbe-assisted phytoextraction of Ni contaminated soils.
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Received: 13 April 2015
Published: 24 November 2015
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