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| Overexpression of LcCHYB to Enhance the Tolerance to Oxidative Stress of Eustoma Grandiflorum |
| JIN Chao1, LIU Jie1,2, JI Jing1, WANG Gang1, CAO Hai-yan1,2, WU Jiang1,2 |
1. School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, China;
2. School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China |
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Abstract Reactive Oxygen Species was accumulated when the plant was under the abiotic stress, which damaged the cell structure and proteins. Carotenoids are non-enzymatic antioxidants, playing an important role to enhance the tolerance to oxidative stress in plants. β-carotene hydroxylase was a key enzyme in carotenoid biosynthesis pathway and coded by CHYB gene. In this study, the LcCHYB was overexpressed in transgenic Eustoma grandiflorum, the results shown When the plants were exposed to 10% H2O2, the Fv/Fm, ФPSⅡ, SOD and POD increased significantly, the content of total carotenoids, Zeaxanthin and Xanthophyll cycle were also enhanced significantly in transgenic line compare to the wild type. Overexpression of LcCHYB enhanced the tolerance to oxidative stress in Eustoma grandiflorum was proved.
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Received: 21 May 2014
Published: 25 January 2015
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