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| Mutagenic Screening Method of Isochrysis zhangjiangensis by Atmospheric and Room Temperature Plasmas |
| CAO Xu-peng, AI Jiang-ning, LIU Ya-nan, ZHOU Jian-nan, WU Pei-chun, XUE Song |
| Marine Bioengineering Group, Dalian Institute of Chemical and Physics, Chinese Academy of Sciences, Dalian 116023, China |
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Abstract To select algal strains with fast growth rate or high oil content, Isochrysis zhangjiangensis is mutated with atmospheric and room temperature plasmas (ARTP). The mutants are screened by well and flask at room temperature ordinary light conditions and stress conditions, such as high temperature ordinary light, high light ordinary temperature. An integrated screening method is set up. Through 600 ml bioreactor cultivated evaluation, the rate of the detection of mutants is 0.7% at room temperature ordinary light conditions, 1.1% at stress conditions, 0.6% through once mutagenesis and 1.2% through secondary mutagenesis. The results show that screening rate is high at secondary mutagenesis and stress conditions. It is easier to obtain mutants with character change.
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Received: 04 August 2014
Published: 25 December 2014
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