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| Studies on the Measurement of Viable Biomass in the Optimization of Rifamycins SV Fermentation Process |
| LIU Ai-jun1, SHI Shou-kun1, LI Lan2, WANG Ping2,3, WANG Wei3, JIA Jun-qiao1, WANG Ze-jian2, LI Hai-dong1, ZHUANG Ying-ping2, ZHANG Si-liang2 |
1. Hebei Xingang Pharmaceutical Co., Ltd., Shijiazhuang 051530, China;
2. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China;
3. Hebei Medical University Xishan Department, Shijiazhuang 050017, China |
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Abstract The amount of viable biomass is an important physiological parameter, which is correlated with the cell growth, metabolism and productivity during the rifamycins SV fermentation process. The measurement of viable biomass was studied employing on-line Biomass Monitor. It was able to utilize the dielectric properties of cells, and the capacitance measurement was correlated well with the viable biomass concentration excluding the interference of solid materials. At the same time, there was close connections among the capacitance measurement, OUR, CER and mycelial morphology. The diauxic growth phenomenon in the former fermentation was detected using capacitance detection, and the slow-release nitrogen source soyben powder instead of the original expensive fast-release nitrogen source of peptone was used for rifamycins SV fermentation, successfully eliminated the diauxic growth caused by nitrogen source using the conversion of stagnation, the physiological parameters of OUR and CER reached and maintained at 14.8 and 15.3 mmol/L/h at early growth phase, significantly higher than that under peptone conditions for only 8.6 and 11.3 mmol/L/h, which promote the continued higher rifamycin SV biosynthesis, the fermentation titer reached to 5969 + 19 U/ml, which was 18.7% higher than that of control (5030 + 17 U/ml).
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Received: 08 July 2014
Published: 25 October 2014
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Cite this article:
LIU Ai-jun, SHI Shou-kun, LI Lan, WANG Ping, WANG Wei, JIA Jun-qiao, WANG Ze-jian, LI Hai-dong, ZHUANG Ying-ping, ZHANG Si-liang. Studies on the Measurement of Viable Biomass in the Optimization of Rifamycins SV Fermentation Process. China Biotechnology, 2014, 34(10): 73-78.
URL:
https://manu60.magtech.com.cn/biotech/10.13523/j.cb.20141012 OR https://manu60.magtech.com.cn/biotech/Y2014/V34/I10/73
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