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| In Vivo Imaging of Near-infrared Fluorescent Protein in Skeletal Muscle of Mice Mediated by Recombinant Adeno-associated Virus |
| TIAN Ting1,2,3, CHANG Jian2, ZHANG Xin2, JIANG Chen-yu2, ZHANG Yun-hai2, LIU Xiao-mei2, ZHANG Chun2 |
1. Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun 130033, China;
2. Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China;
3. University of Chinese Academy of Sciences, Beijing 100049, China |
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Abstract Near-infrared fluorescent protein (iRFP) which effectively reduces light absorption and scattering in animal tissues is suitable for living animal deep tissue imaging. Eukaryotic expression vector, pAAV-iRFP713, near-infrared fluorescent protein 713 (iRFP) was constructed. Recombinant adeno-associated virus, rAAV-iRFP713, was packaged. In vitro infection of cancer cells with rAAV-iRFP713 resulted in the strong expression of near-infrared fluorescent protein after 48h. rAAV-iRFP713 was injected into skeletal muscle of mice. Strong in vivo fluorescence signal was detected with the infrared fluorescence imaging system 48h after injection of rAAV-iRFP713. The results suggest that rAAV could deliver near-infrared fluorescent reporter gene in vitro and in vivo and iRFPs might be favorable fluorescent molecular labeling agents for living dynamic animal imaging.
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Received: 12 August 2014
Published: 25 October 2014
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