|
|
|
| rhKGF2-EGFP Fusion Expression and Alcohol Liposome Preparation |
| ZHAO Hai-yang1,2,3, WANG Ze2,3,4, HUANG Peng-huang2,3,4, TIAN Hai-shan2,3, LI Xiao-kun1,2,4, YANG Shu-lin1 |
1. School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China;
2. School of Pharmacy, Wenzhou Medical University, Wenzhou 325035, China;
3. Zhejiang Gelusite Biology Technology Co. Ltd, Wenzhou 325000, China;
4. School of Life Science, Jilin Agriculture University, Changchun 130021, China |
|
|
|
Abstract Numerous studies proved, recombinant human keratinocyte growth factor play the significant role in promoting hair growth, Successfully build rhKGF2-EGFP (enhanced green fluorescent protein) fusion protein expression vector, and gain pure rhKGF2-EGFP fusion protein. Rehydrated lyophilized protein pure liposomes prepared by rhKGF2-EGFP. The reuse of the characters of liposomes beneficial to the skin penetration and sustained release characteristics, and EGFP fluorescence characteristics, liposomes rhKGF2-EGFP hair-growth related skin permeability study.
|
|
Received: 08 July 2014
Published: 25 October 2014
|
|
|
|
|
|
[1] Igarashi M,Finch P W,Aaronson S A.Characterization of recombinant human fibroblast growth factor(FGF)-10 reveals functional similarities with keratinocyte growth factor(FGF-7).Biol Chem,1998,273(21):13230-13235.
[2] Lu W,Luo Y,Kan M,et al.Fibroblast growth factor-10.A second candidate stromal to epithelial cell and romedin in prostate.Biol Chem,1999,274(18):12827-12834.
[3] Beer H D,Vindevoghel L,Gait M J,et al.Fibroblast growth factor (FGF) receptor 1-IIIb is a naturally occurring functional receptor for FGFs that is preferentially expressed in the skin and the brain.Biol Chem,2000,275(21):16091-16097.
[4] Pirvola U, Spencer-Dene B, Xing-Qun L, et al.FGF/FGFR-2(IIIb) signaling is essential for inner ear morphogenesis.Neurosci,2000,20(16):6125-6134.
[5] Ainbinder D, Paolino D, Fresta M, et al. Drug delivery applications with ethosomes. J Biomed Nanotechnol, 2010,6(5):558-568.
[6] Sinico C, Fadda A M. Vesicular carriers for dermal drug delivery. Expert Opin Drug Deliv, 2009,6(8):813-825.
[7] Bendas E R, Tadros M I. Enhanced transdermal delivery of salbutamol sulfate via ethosomes. AAPS Pharm Sci Tech, 2007,8(4):107-112.
[8] Prausnitz M R,Mitragotri S,Langer R.Current status and future potential of transdermal drug delivery.Nat Rev Drug Discov, 2004,3:115-124.
[9] Mitragotri S.Current status and future prospects of needle-free liquid jetinjectors.Nat Rev Drug Discov, 2006,5:543-548.
[10] 王泽,黄鹏煌,赵海洋,等.重组人角质细胞生长因子2对实验秃毛大鼠的毛发再生作用.中国药理学通报,2012,(12):1741-1746. Wang Z,Huang P H,Zhao H Y,et al.Effects of recombinant human keratinocyte growth factor 2 on hair regeneration in experimental bald rats.Chinese Pharmacological Bulletin,2012,(12):1741-1746.
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
